IL12 p70 Human Elispot Kit (Reagents for 5 x 96 tests, without plates) (ab46552)

Overview

  • Product nameIL12 p70 Human Elispot Kit (Reagents for 5 x 96 tests, without plates)See all IL12 p70 kits ...
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Cell culture extracts
  • Assay typeSandwich (qualitative)
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Elispot assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

    This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

    Principal:

    After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.

     

    Recognizes natural human IL12 Specific to p70 heterodimer

  • Tested applicationsMeasuring secreted proteins from cellsmore details
  • PlatformReagents

Properties

  • RelevanceInterleukin 12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 35 kD cytokine receptor like subunit encoded by IL12A gene, and a 40 kD subunit encoded by IL12B. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. It is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.
  • Cellular localizationSecreted
  • Alternative names
    • CLMF p35
    • CLMF p40
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit
    • IL 12A
    • IL 12B
    • IL12 subunit p35
    • IL12 subunit p40
    • Interleukin 12 alpha chain
    • Interleukin 12 beta chain
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISPOT Use at an assay dependent dilution.

References for IL12 p70 Human Elispot Kit (Reagents for 5 x 96 tests, without plates) (ab46552)

ab46552 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. Neither of the Elispot kits ab46552 or ab46574 are currently shipped with milk. The customer can either use BSA or any commercially available dry milk for the blocking step in the protocols for these kits. I hope this helps...

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Thank you for your call today and for your question about this kit. I apologize again for the confusion about the 96-well plates being included in the kit. I will request that we remove the plates from the components list to avoid this problem in the f...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"