Overview

  • Product nameAnti-IL12RB2 antibody
    See all IL12RB2 primary antibodies
  • Description
    Mouse polyclonal to IL12RB2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human)

  • Positive control
    • IL12RB2 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab67365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 97 kDa).

Target

  • FunctionReceptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.
  • Tissue specificityIsoform 2 is expressed at similar levels in both naive and activated T-cells.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 5 fibronectin type-III domains.
  • Developmental stageMaximum levels in Th1 cells between day 3 and day 8 of activation.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    modifications
    On IL12 binding, phosphorylated on C-terminal tyrosine residues by JAK2. Phosphorylation on Tyr-800 is required for STAT4 binding and activation, and for SOCS3 binding.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • I12R2_HUMAN antibody
    • IL-12 receptor subunit beta-2 antibody
    • IL-12R subunit beta-2 antibody
    • IL-12R-beta-2 antibody
    • IL-12RB2 antibody
    • IL12 receptor beta 2 antibody
    • IL12R beta2 antibody
    • IL12RB2 antibody
    • Interleukin 12 receptor beta 2 antibody
    • Interleukin 12 receptor beta 2 chain antibody
    • Interleukin-12 receptor subunit beta-2 antibody
    • RP11 102M16.1 antibody
    see all

Anti-IL12RB2 antibody images

  • All lanes : Anti-IL12RB2 antibody (ab67365) at 1/500 dilution

    Lane 1 : IL12RB2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 97 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 140 kDa. We are unsure as to the identity of these extra bands.

References for Anti-IL12RB2 antibody (ab67365)

ab67365 has not yet been referenced specifically in any publications.

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