Anti-IL-12RB2 antibody (ab96097)
Key features and details
- Rabbit polyclonal to IL-12RB2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-12RB2 antibody
See all IL-12RB2 primary antibodies -
Description
Rabbit polyclonal to IL-12RB2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human IL-12RB2 aa 323-665.
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Positive control
- 293T, H1299, HeLaS3, Molt 4, Raji; A431 or HepG2 whole cell lysate (ab7900)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 97 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 97 kDa. |
Target
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Function
Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production. -
Tissue specificity
Isoform 2 is expressed at similar levels in both naive and activated T-cells. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains. -
Developmental stage
Maximum levels in Th1 cells between day 3 and day 8 of activation. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Post-translational
modificationsOn IL12 binding, phosphorylated on C-terminal tyrosine residues by JAK2. Phosphorylation on Tyr-800 is required for STAT4 binding and activation, and for SOCS3 binding. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3595 Human
- Omim: 601642 Human
- SwissProt: Q99665 Human
- Unigene: 479347 Human
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Alternative names
- I12R2_HUMAN antibody
- IL-12 receptor subunit beta-2 antibody
- IL-12R subunit beta-2 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab96097 has been referenced in 1 publication.
- Hackett TL et al. Fibroblast signal transducer and activator of transcription 4 drives cigarette smoke-induced airway fibrosis. Am J Respir Cell Mol Biol 51:830-9 (2014). PubMed: 24922586