Anti-IL13 antibody [13A] (Phycoerythrin) (ab95576)

Overview

  • Product nameAnti-IL13 antibody [13A] (Phycoerythrin)
    See all IL13 primary antibodies
  • Description
    Rat monoclonal [13A] to IL13 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length Mouse IL13 protein.

  • Positive control
    • BALB/c splenocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab95576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.25µg for 105-8 cells. ab154449-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • Involvement in diseaseAllergic rhinitis
  • Sequence similaritiesBelongs to the IL-4/IL-13 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Allergic rhinitis antibody
    • ALRH antibody
    • BHR1 antibody
    • Bronchial hyperresponsiveness 1 (bronchial asthma) antibody
    • IL 13 antibody
    • IL-13 antibody
    • Il13 antibody
    • IL13_HUMAN antibody
    • Interleukin 13 antibody
    • Interleukin-13 antibody
    • MGC116786 antibody
    • MGC116788 antibody
    • MGC116789 antibody
    • NC30 antibody
    • P600 antibody
    see all

Anti-IL13 antibody [13A] (Phycoerythrin) images

  • ab95576 at 0.125 µg/test staining IL13 by Flow Cytometry.
    BALB/c splenocytes were cultured for 3 days with plate-bound Anti-Mouse CD3e, soluble Anti-Mouse CD28, Mouse IL2 Recombinant Protein and Mouse IL4 Recombinant Protein. Cells were then harvested and restimulated with PMA and Ionomycin in the presence of monensin for 5 hours. Following restimulation, cells were harvested, surface stained with Anti-Mouse CD4 FITC, fixed and permeabilized, then stained with 0.125 µg of ab95576 (1). The ligand blocking control demonstrates that pre-blocking ab95576 with 1.0 µg of Mouse IL13 Recombinant Protein prior to staining abrogates specific mouse IL13 staining (2).

References for Anti-IL13 antibody [13A] (Phycoerythrin) (ab95576)

ab95576 has not yet been referenced specifically in any publications.

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