Overview

  • Product name
  • Description
    Rabbit polyclonal to IL13
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to C terminal amino acids 25-146 of Human IL13 (BC096139)

  • Positive control
    • Human fetal spleen lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab106732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 16 kDa.
IHC-P 1/100 - 1/500.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • Involvement in disease
    Allergic rhinitis
  • Sequence similarities
    Belongs to the IL-4/IL-13 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Allergic rhinitis antibody
    • ALRH antibody
    • BHR1 antibody
    • Bronchial hyperresponsiveness 1 (bronchial asthma) antibody
    • IL 13 antibody
    • IL-13 antibody
    • Il13 antibody
    • IL13_HUMAN antibody
    • Interleukin 13 antibody
    • Interleukin-13 antibody
    • MGC116786 antibody
    • MGC116788 antibody
    • MGC116789 antibody
    • NC30 antibody
    • P600 antibody
    see all

Images

  • Anti-IL13 antibody (ab106732) at 1/1000 dilution + Human fetal spleen lysate

    Predicted band size : 16 kDa
  • ab106732 staining IL13 in human intestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde, a heat mediated antigen retrieval step was performed using citrate buffer pH 6, samples were then incubated with ab106732 at a 1/800 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP polymer conjugated goat anti-rabbit/mouse polyclonal.

    See Abreview

  • ab106732 staining IL13 in murine colon tissue by Immunohistochemistry (Frozen sections).

    Tissue was fixed with paraformaldehyde and blocked with 5% serum for 20 hours at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA in PBS) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

  • Anti-IL13 antibody (ab106732) at 1/1000 dilution + Mouse liver tissue lysate at 100 µg

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

References

This product has been referenced in:
  • Nakama T  et al. Therapeutic Effect of Novel Single-Stranded RNAi Agent Targeting Periostin in Eyes with Retinal Neovascularization. Mol Ther Nucleic Acids 6:279-289 (2017). Read more (PubMed: 28325294) »
  • Ma Y  et al. Toll-like receptor (TLR) 2 and TLR4 differentially regulate doxorubicin induced cardiomyopathy in mice. PLoS One 7:e40763 (2012). IHC-P ; Mouse . Read more (PubMed: 22808256) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Resin sections)
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil
Username

Mr. Ali Hosseini

Verified customer

Submitted Oct 27 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Mouse Tissue sections (Uterus)
Specification
Uterus
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

Submitted Nov 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Mouse Tissue sections (uteurs)
Specification
uteurs
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Varkha Agrawal

Verified customer

Submitted Sep 24 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Sheep Tissue sections (Lung tissue and lymph node)
Specification
Lung tissue and lymph node
Fixative
Acetone
Permeabilization
No
Blocking step
(agent) for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver whole extract)
Loading amount
100 µg
Specification
Liver whole extract
Gel Running Conditions
Reduced Denaturing (14% Tris-glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Colon)
Specification
Colon
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 05 2012

DISCOUNT CODEs

ab106732 : 00000

ab115672 : 00000

Expiration date: 16. November 2012

I can confirm that the discount codescan be redeemed with Sapphire Biosciences. Predicted species are not covered by the Abpromis...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (intestine)
Specification
intestine
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH6, 20 min, 100C
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Oct 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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