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Our Abpromise guarantee covers the use of ab106732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 16 kDa.|
|IHC-P||1/100 - 1/500.|
|IHC-Fr||Use at an assay dependent concentration.|
ab106732 staining IL13 in human intestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde, a heat mediated antigen retrieval step was performed using citrate buffer pH 6, samples were then incubated with ab106732 at a 1/800 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP polymer conjugated goat anti-rabbit/mouse polyclonal.