The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Mouse IL13.
Use a concentration of 0.08 - 0.1 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of IL13 (10.0 ng/ml)
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL13 (Biotin) (ab83162). Using ab83162 as the detection antibody and ab109815 as the capture antibody, the detection limit for recombinant Mouse IL13 is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 15 kDa. The detection limit for recombinant Mouse IL13 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionCytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
Involvement in diseaseAllergic rhinitis
Sequence similaritiesBelongs to the IL-4/IL-13 family.