Overview

  • Product name
  • Description
    Rabbit polyclonal to IL13
  • Tested applications
    Suitable for: WB, ELISA, Neutralising, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hIL-13 (human Interleukin-13)

  • Positive control
    • IHC-P: human testis tissue sections

Properties

Applications

Our Abpromise guarantee covers the use of ab9576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-13 (assuming 100 µl/well antibody solution).
Neutralising Use a concentration of 0.12 - 0.13 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hIL-13 (2.0 ng/ml).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • Involvement in disease
    Allergic rhinitis
  • Sequence similarities
    Belongs to the IL-4/IL-13 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Allergic rhinitis antibody
    • ALRH antibody
    • BHR1 antibody
    • Bronchial hyperresponsiveness 1 (bronchial asthma) antibody
    • IL 13 antibody
    • IL-13 antibody
    • Il13 antibody
    • IL13_HUMAN antibody
    • Interleukin 13 antibody
    • Interleukin-13 antibody
    • MGC116786 antibody
    • MGC116788 antibody
    • MGC116789 antibody
    • NC30 antibody
    • P600 antibody
    see all

Images

  • All lanes : Anti-IL13 antibody (ab9576) at 1/3000 dilution

    Lane 1 : E. coli expressed human recombinant protein IL13 from supplier 1 at 0.2 µg
    Lane 2 : E. coli expressed human recombinant protein IL13 from supplier 1 at 0.3 µg
    Lane 3 : E. coli expressed human recombinant protein IL13 from supplier 2 at 0.2 µg
    Lane 4 : E. coli expressed human recombinant protein IL13 from supplier 2 at 0.3 µg
    Lane 5 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.2 µg
    Lane 6 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.3 µg
    Lane 7 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.4 µg

    Secondary
    HRP conjugated goat anti-rabbit at 1/2000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Observed band size : 10 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa (possible dimer).

    Exposure time : 5 minutes

    This image is courtesy of an Abreview submitted by Miss Fenny Chong

    15% denaturing gel.  Blot was blocked with 2% milk for 16 hours at 4°C and incubated with the primary antibody for 1 hour at 25°C.

    See Abreview

  • IHC image of IL13 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9576, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Calibration curve of a sandwich assay for IL13 using ab9576 as a detection antibody at a concentration of 0.5μg/ml.

References

ab9576 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Ventana Protease 1
Permeabilization
No
Specification
Lung
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (E.coli expressed commercial protein)
Loading amount
0.3 µg
Specification
E.coli expressed commercial protein
Gel Running Conditions
Non-reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Username

Miss. Fenny Chong

Verified customer

Submitted Mar 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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