Highly pure (>98%) recombinant hIL-13 (human Interleukin-13)
IHC-P: human testis tissue sections
FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPBS, pH 7.4, no preservative, sterile filtered
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.2 µg/ml.
Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-13 (assuming 100 µl/well antibody solution).
Use a concentration of 0.12 - 0.13 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hIL-13 (2.0 ng/ml).
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionCytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
Involvement in diseaseAllergic rhinitis
Sequence similaritiesBelongs to the IL-4/IL-13 family.
All lanes : Anti-IL13 antibody (ab9576) at 1/3000 dilution
Lane 1 : E. coli expressed human recombinant protein IL13 from supplier 1 at 0.2 µg Lane 2 : E. coli expressed human recombinant protein IL13 from supplier 1 at 0.3 µg Lane 3 : E. coli expressed human recombinant protein IL13 from supplier 2 at 0.2 µg Lane 4 : E. coli expressed human recombinant protein IL13 from supplier 2 at 0.3 µg Lane 5 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.2 µg Lane 6 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.3 µg Lane 7 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.4 µg
Secondary HRP conjugated goat anti-rabbit at 1/2000 dilution developed using the ECL technique
IHC image of IL13 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9576, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-IL13 antibody (ab9576)
has not yet been referenced specifically in any publications.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"