Our Abpromise guarantee covers the use of ab55275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 44 kDa.|
|IHC-P||Use a concentration of 1 µg/ml.|
|ICC||Use a concentration of 1 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-Fr||Use a concentration of 1 µg/ml.|
ab55275 staining IL13 rexeptor alpha 2 in Rabbit ear wound exudate tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 100% Protein block (serum free) for 30 minutes at 37°C; antigen retrieval was enzymatic. Samples were incubated with primary antibody (1µg/ml) for 1 hour at 37°C. An undiluted HRP-conjugated Mouse anti-mouse IgG polyclonal was used as the secondary antibody.