Overview

  • Product name
  • Description
    Rabbit polyclonal to IL15
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, African green monkey
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 1-50 (RISKPHLRSI SIQCYLCLLL NSHFLTEAGI HVFILGCFSA GLPKTEANWV) of Human IL15, NP_751914.

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 10, 15 kDa (predicted molecular weight: 18 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-IL15 antibody (ab85010) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 18 kDa
    Observed band size : 10,15 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Shi L  et al. miR-15b is Downregulated in Myasthenia Gravis Patients and Directly Regulates the Expression of Interleukin-15 (IL-15) in Experimental Myasthenia Gravis Mice. Med Sci Monit 21:1774-80 (2015). WB . Read more (PubMed: 26087886) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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