The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 0.5 µg for 106 cells.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 28 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for interleukin-15. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves STAT3, STAT5, STAT6, JAK2 (By similarity) and SYK.
Tissue specificityIsoform 1, isoform 3, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9 are widely expressed. Expressed in fetal brain with higher expression in the hippocampus and cerebellum than in cortex and thalamus. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.
Post-translational modificationsA soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE (By similarity). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor. Phosphorylated by activated SYK. N-glycosylated and O-glycosylated.
Cellular localizationSecreted > extracellular space; Membrane. Nucleus membrane. Mainly found associated with the nuclear membrane and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle membrane. Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane.
ab91270 staining IL15RA in normal Human PBMCs by Flow cytometry.
Normal Human PBMCs were isolated, and cultured in standard conditions for 3 hours. Non adherent cells were removed from culture, and remaining cells were stimulated with LPS and IFN-? for 24 hours.
Open/green histogram; stained with 0.25 µg of Purified Mouse IgG2b Isotype Control
Closed/blue histogram; stained with 0.25 µg of ab91270.
Detection utilised Biotin Anti-Mouse IgG and SAv-PE.
Cells in the large scatter population were used for analysis.
References for Anti-IL15RA antibody [JM7A4] (ab91270)
This product has been referenced in:
Vámosi G et al. IL-2 and IL-15 receptor alpha-subunits are coexpressed in a supramolecular receptor cluster in lipid rafts of T cells. Proc Natl Acad Sci U S A101:11082-7 (2004).
Read more (PubMed: 15263076) »