Overview

  • Product nameAnti-IL15RA antibody [JM7A4]
    See all IL15RA primary antibodies
  • Description
    Mouse monoclonal [JM7A4] to IL15RA
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    E. coli protein

  • Positive control
    • LPS and IFN gamma stimulated PBMCs

Properties

Applications

Our Abpromise guarantee covers the use of ab91270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
Flow Cyt
  • Application notesFlow Cyt: Use 0.5 µg for 106 cells.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 28 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for interleukin-15. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves STAT3, STAT5, STAT6, JAK2 (By similarity) and SYK.
    • Tissue specificityIsoform 1, isoform 3, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9 are widely expressed. Expressed in fetal brain with higher expression in the hippocampus and cerebellum than in cortex and thalamus. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.
    • Sequence similaritiesContains 1 Sushi (CCP/SCR) domain.
    • Post-translational
      modifications
      A soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE (By similarity). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor.
      Phosphorylated by activated SYK.
      N-glycosylated and O-glycosylated.
    • Cellular localizationSecreted > extracellular space; Membrane. Nucleus membrane. Mainly found associated with the nuclear membrane and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle membrane. Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AA690181 antibody
      • CD215 antibody
      • I15RA_HUMAN antibody
      • IL 15R alpha antibody
      • IL-15 receptor subunit alpha antibody
      • IL-15R-alpha antibody
      • IL-15RA antibody
      • Il15ra antibody
      • Interleukin 15 receptor alpha antibody
      • Interleukin 15 receptor subunit alpha antibody
      • MGC104179 antibody
      • sIL-15 receptor subunit alpha antibody
      • sIL-15R-alpha antibody
      • sIL-15RA antibody
      • Soluble interleukin 15 receptor subunit alpha antibody
      • Soluble interleukin-15 receptor subunit alpha antibody
      see all

    Anti-IL15RA antibody [JM7A4] images

    • ab91270 staining IL15RA in normal Human PBMCs by Flow cytometry.
      Normal Human PBMCs were isolated, and cultured in standard conditions for 3 hours. Non adherent cells were removed from culture, and remaining cells were stimulated with LPS and IFN-? for 24 hours.
      Open/green histogram; stained with 0.25 µg of Purified Mouse IgG2b Isotype Control
      Closed/blue histogram; stained with 0.25 µg of ab91270.
      Detection utilised Biotin Anti-Mouse IgG and SAv-PE.
      Cells in the large scatter population were used for analysis.

    References for Anti-IL15RA antibody [JM7A4] (ab91270)

    This product has been referenced in:
    • Vámosi G  et al. IL-2 and IL-15 receptor alpha-subunits are coexpressed in a supramolecular receptor cluster in lipid rafts of T cells. Proc Natl Acad Sci U S A 101:11082-7 (2004). Read more (PubMed: 15263076) »
    • Dubois S  et al. IL-15Ralpha recycles and presents IL-15 In trans to neighboring cells. Immunity 17:537-47 (2002). Read more (PubMed: 12433361) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"