Anti-IL15RA antibody [MM0369-5M53] (ab89894)

Overview

  • Product nameAnti-IL15RA antibody [MM0369-5M53]
    See all IL15RA primary antibodies
  • Description
    Mouse monoclonal [MM0369-5M53] to IL15RA
  • Specificityab89894 detects IL15RA.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to the extracellular domain of Human IL15RA.

Properties

Applications

Our Abpromise guarantee covers the use of ab89894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa. WB ab89894 has been tested only against the immunogen and negative results were obtained in ELISA against mouse IL15RA.

Target

  • FunctionReceptor for interleukin-15. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves STAT3, STAT5, STAT6, JAK2 (By similarity) and SYK.
  • Tissue specificityIsoform 1, isoform 3, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9 are widely expressed. Expressed in fetal brain with higher expression in the hippocampus and cerebellum than in cortex and thalamus. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.
  • Sequence similaritiesContains 1 Sushi (CCP/SCR) domain.
  • Post-translational
    modifications
    A soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE (By similarity). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor.
    Phosphorylated by activated SYK.
    N-glycosylated and O-glycosylated.
  • Cellular localizationSecreted > extracellular space; Membrane. Nucleus membrane. Mainly found associated with the nuclear membrane and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle membrane. Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA690181 antibody
    • CD215 antibody
    • I15RA_HUMAN antibody
    • IL 15R alpha antibody
    • IL-15 receptor subunit alpha antibody
    • IL-15R-alpha antibody
    • IL-15RA antibody
    • Il15ra antibody
    • Interleukin 15 receptor alpha antibody
    • Interleukin 15 receptor subunit alpha antibody
    • MGC104179 antibody
    • sIL-15 receptor subunit alpha antibody
    • sIL-15R-alpha antibody
    • sIL-15RA antibody
    • Soluble interleukin 15 receptor subunit alpha antibody
    • Soluble interleukin-15 receptor subunit alpha antibody
    see all

References for Anti-IL15RA antibody [MM0369-5M53] (ab89894)

ab89894 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Recombinant protein (hIL-15ra recombinant protein transduced into EL ce)
Loading amount 15 µg
Specification hIL-15ra recombinant protein transduced into EL ce
Treatment BME
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Gong Yun

Verified customer

Submitted Jan 10 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"