Overview

  • Product nameAnti-IL16 antibody
    See all IL16 primary antibodies
  • Description
    Rabbit polyclonal to IL16
  • Tested applicationsSuitable for: ELISA, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hIL-16 (human Interleukin-16)

Properties

  • FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage bufferPBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • Isotypeunknown
  • Light chain typeunknown
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab9563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Neutralising
WB
  • Application notesNeutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hIL-16 (4.2 ng/ml), a concentration of 0.07 - 0.012 µg/ml of this antibody is required.

    ELISA:To detect hIL-16 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-16.

    Western Blot:To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • FunctionInterleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
      Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
      Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
    • Tissue specificityIsoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation.
    • Sequence similaritiesContains 4 PDZ (DHR) domains.
    • Post-translational
      modifications
      Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.
    • Cellular localizationCytoplasm; Cytoplasm. Nucleus and Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • HGNC:5980 antibody
      • HsT19289 antibody
      • IL-16 antibody
      • IL16 antibody
      • IL16_HUMAN antibody
      • Interleukin 16 precursor antibody
      • Interleukin-16 antibody
      • LCF antibody
      • Lymphocyte chemoattractant factor antibody
      • Neuronal interleukin 16 antibody
      • NIL16 antibody
      • prIL 16 antibody
      • PrIL16 antibody
      • Prointerleukin 16 antibody
      see all

    References for Anti-IL16 antibody (ab9563)

    This product has been referenced in:
    • Ahn DS  et al. Secretion of IL-16 through TNFR1 and calpain-caspase signaling contributes to MRSA pneumonia. Mucosal Immunol N/A:N/A (2014). Read more (PubMed: 24736233) »

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample Mouse Cell (Hepatocyte)
    Specification Hepatocyte
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 21 2014

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Non-reduced Denaturing (15%)
    Sample Mouse Cell lysate - whole cell (primary hepatoctyes)
    Specification primary hepatoctyes
    Blocking step I-Block(Applied biosystems) as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 09 2013


    The immunogen sequence of ab9563 is:
    MPDLNSSTDS AASASAASDV SVESTAEATV CTVTLEKMSA GLGFSLEGGK GSLHGDKPLT INRIFKGAAS EQSETVQPGD EILQLGGTAM QGLTRFEAWN IIKALPDGPV TIVIRRKSLQ SKETTAAGDS

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"