Overview

  • Product name
  • Description
    Rabbit polyclonal to IL16
  • Tested applications
    Suitable for: ELISA, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hIL-16 (human Interleukin-16)

Properties

  • Form
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Neutralising
WB
  • Application notes
    Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hIL-16 (4.2 ng/ml), a concentration of 0.07 - 0.012 µg/ml of this antibody is required.

    ELISA:To detect hIL-16 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-16.

    Western Blot:To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • Function
      Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
      Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
      Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
    • Tissue specificity
      Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation.
    • Sequence similarities
      Contains 4 PDZ (DHR) domains.
    • Post-translational
      modifications
      Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.
    • Cellular localization
      Cytoplasm; Cytoplasm. Nucleus and Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • HGNC:5980 antibody
      • HsT19289 antibody
      • IL-16 antibody
      • IL16 antibody
      • IL16_HUMAN antibody
      • Interleukin 16 precursor antibody
      • Interleukin-16 antibody
      • LCF antibody
      • Lymphocyte chemoattractant factor antibody
      • Neuronal interleukin 16 antibody
      • NIL16 antibody
      • prIL 16 antibody
      • PrIL16 antibody
      • Prointerleukin 16 antibody
      see all

    References for Anti-IL16 antibody (ab9563)

    This product has been referenced in:
    • Ahn DS  et al. Secretion of IL-16 through TNFR1 and calpain-caspase signaling contributes to MRSA pneumonia. Mucosal Immunol N/A:N/A (2014). Read more (PubMed: 24736233) »

    See 1 Publication for this product

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample
    Mouse Cell (Hepatocyte)
    Specification
    Hepatocyte
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 21 2014

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Non-reduced Denaturing (15%)
    Sample
    Mouse Cell lysate - whole cell (primary hepatoctyes)
    Specification
    primary hepatoctyes
    Blocking step
    I-Block(Applied biosystems) as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 09 2013


    The immunogen sequence of ab9563 is:
    MPDLNSSTDS AASASAASDV SVESTAEATV CTVTLEKMSA GLGFSLEGGK GSLHGDKPLT INRIFKGAAS EQSETVQPGD EILQLGGTAM QGLTRFEAWN IIKALPDGPV TIVIRRKSLQ SKETTAAGDS

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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