The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
FunctionLigand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (PubMed:8676080).
Tissue specificityRestricted to activated memory T-cells.
Sequence similaritiesBelongs to the IL-17 family.
Post-translational modificationsFound both in glycosylated and nonglycosylated forms.
Anti-IL17 antibody (ab77171) at 1 µg/ml + Recombinant human IL17 protein (ab73202) at 0.01 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
References for Anti-IL17 antibody (ab77171)
This product has been referenced in:
Hou C et al. Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines. Int J Mol Med38:713-20 (2016).
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