Overview

  • Product name
  • Description
    Rabbit polyclonal to IL17
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human IL17.

    (Peptide available as ab88219.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Thymus Tissue Lysate; U937 Whole Cell Lysate; THP1 Whole Cell Lysate; Raji Whole Cell Lysate; Ramos Whole Cell Lysate; Lymph node Tissue Lysate; Tonsil Tissue Lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

Target

  • Function
    Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (PubMed:8676080).
  • Tissue specificity
    Restricted to activated memory T-cells.
  • Sequence similarities
    Belongs to the IL-17 family.
  • Post-translational
    modifications
    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CTLA 8 antibody
    • CTLA-8 antibody
    • CTLA8 antibody
    • Cytotoxic T lymphocyte associated antigen 8 antibody
    • Cytotoxic T lymphocyte associated protein 8 antibody
    • Cytotoxic T lymphocyte associated serine esterase 8 antibody
    • Cytotoxic T-lymphocyte-associated antigen 8 antibody
    • IL 17A antibody
    • IL-17 antibody
    • IL-17A antibody
    • IL17 antibody
    • IL17_HUMAN antibody
    • Il17a antibody
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) antibody
    • Interleukin 17A antibody
    • Interleukin-17A antibody
    • Interleukin17 antibody
    • Interleukin17A antibody
    • OTTHUMP00000016597 antibody
    • OTTMUSP00000046003 antibody
    see all

Images

  • All lanes : Anti-IL17 antibody (ab77171) at 1 µg/ml

    Lane 1 : Human thymus tissue lysate - total protein (ab30146)
    Lane 2 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
    Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 5 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 6 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 7 : Tonsil (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 18 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Anti-IL17 antibody (ab77171) at 1 µg/ml + Recombinant human IL17 protein (ab73202) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References

This product has been referenced in:
  • Hou C  et al. Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines. Int J Mol Med 38:713-20 (2016). WB ; Mouse . Read more (PubMed: 27460435) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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