Overview

  • Product nameAnti-IL17 antibody
    See all IL17 primary antibodies
  • Description
    Rabbit polyclonal to IL17
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to Human IL17 (internal sequence). ab79056 was raised against a 19 amino acid synthetic peptide from near the center of human IL-17A.
    Database link: Q16552
    (Peptide available as ab190733)

  • Positive control
  • General notes

    The blocking peptide is available as ab190773

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: 99.98% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionLigand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (PubMed:8676080).
  • Tissue specificityRestricted to activated memory T-cells.
  • Sequence similaritiesBelongs to the IL-17 family.
  • Post-translational
    modifications
    Found both in glycosylated and nonglycosylated forms.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CTLA 8 antibody
    • CTLA-8 antibody
    • CTLA8 antibody
    • Cytotoxic T lymphocyte associated antigen 8 antibody
    • Cytotoxic T lymphocyte associated protein 8 antibody
    • Cytotoxic T lymphocyte associated serine esterase 8 antibody
    • Cytotoxic T-lymphocyte-associated antigen 8 antibody
    • IL 17A antibody
    • IL-17 antibody
    • IL-17A antibody
    • IL17 antibody
    • IL17_HUMAN antibody
    • Il17a antibody
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) antibody
    • Interleukin 17A antibody
    • Interleukin-17A antibody
    • Interleukin17 antibody
    • Interleukin17A antibody
    • OTTHUMP00000016597 antibody
    • OTTMUSP00000046003 antibody
    see all

Anti-IL17 antibody images

  • Anti-IL17 antibody (ab79056) at 1 µg/ml + Recombinant human IL-17 at 0.001 µg

    Predicted band size : 18 kDa
  • ab79056 staining IL17 in Dog lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with Tween-20 (0.05%) and blocked with 10% serum for 2 hours at 24°C; antigen retrieval was by heat mediation with citrate buffer (0.01M). Samples were incubated with primary antibody (1/100 in PBS +10% serum) for 14 hours at 4°C. A Biotin-conjugated Horse pan-specific anti-IgG polyclonal (1/20) was used as the secondary antibody.

    See Abreview

  • Lane 1 : Anti-IL17 antibody (ab79056) at 0.5 µg/ml
    Lane 2 : Anti-IL17 antibody (ab79056) at 1 µg/ml

    Lane 1 : A-20 cell lysate
    Lane 2 : A-20 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 18 kDa
    Observed band size : 18 kDa
    Additional bands at : 22 kDa,28 kDa,32 kDa. We are unsure as to the identity of these extra bands.
    The higher molecular weight bands seen in the Western blot may represent glycosylated IL17.
  • All lanes : Anti-IL17 antibody (ab79056)

    Lane 1 : human thymus tissue lysate at 1 µg/ml
    Lane 2 : human thymus tissue lysate at 2 µg/ml


    Predicted band size : 18 kDa

References for Anti-IL17 antibody (ab79056)

This product has been referenced in:
  • Quaresma JA  et al. T-helper 17 cytokines expression in leprosy skin lesions. Br J Dermatol 173:565-7 (2015). Read more (PubMed: 25495069) »
  • Yoon JH  et al. Phosphorylation status determines the opposing functions of Smad2/Smad3 as STAT3 cofactors in TH17 differentiation. Nat Commun 6:7600 (2015). IHC ; Mouse . Read more (PubMed: 26194464) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 8 minute(s) · Concentration: 25% · Temperature: 37°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrat
Sample Rhesus monkey Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Glutaraldehyde
Username

Ms. Maria Daskalaki

Verified customer

Submitted Nov 13 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: microwave with citrate buffer pH 6.0
Sample Cow Tissue sections (Mammary gland)
Specification Mammary gland
Permeabilization No
Fixative Formaldehyde
Username

Dr. Natalia Salvetti

Verified customer

Submitted Oct 09 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (salivary gland)
Specification salivary gland
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Tris pH=9.0 / 1mM EDTA
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Jan 09 2013

Thank you for your enquiry.

I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications and species listed on the datasheets. Therefore, any IL17 antibody teste...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver whole extract)
Loading amount 100 µg
Specification Liver whole extract
Gel Running Conditions Reduced Denaturing (14% Tris-glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Sep 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Colon)
Specification Colon
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (tonsil)
Specification tonsil
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 6% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 21 2012

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer , pressure cooked for 24 minutes
Permeabilization Yes - 0.3% Triton X-100, 5 min RT
Blocking step (agent) for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

minsha manoharan

Verified customer

Submitted Aug 15 2012

Thank you very much for your interest in ab79056 Anti-IL17 antibody.

To our knowledge, ab79056 has not been tested in sELISA experiment.
The problem with sELISA is that the coating antibody may block the binding site of the detection antib...

Read More



Nous n'avons malheureusement pas de référence particulière décrivant l'expression d'IL-17 par les cellules A20, mais une recherche sur internet vousrenverra une multitude de résultats décrivant ceci.

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