Overview

Properties

  • Form
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-17 (assuming 100 µl/well antibody solution).
Neutralising Use a concentration of 0.1 - 0.18 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hIL-17 (6.0 ng/ml).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (PubMed:8676080).
  • Tissue specificity
    Restricted to activated memory T-cells.
  • Sequence similarities
    Belongs to the IL-17 family.
  • Post-translational
    modifications
    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CTLA 8 antibody
    • CTLA-8 antibody
    • CTLA8 antibody
    • Cytotoxic T lymphocyte associated antigen 8 antibody
    • Cytotoxic T lymphocyte associated protein 8 antibody
    • Cytotoxic T lymphocyte associated serine esterase 8 antibody
    • Cytotoxic T-lymphocyte-associated antigen 8 antibody
    • IL 17A antibody
    • IL-17 antibody
    • IL-17A antibody
    • IL17 antibody
    • IL17_HUMAN antibody
    • Il17a antibody
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) antibody
    • Interleukin 17A antibody
    • Interleukin-17A antibody
    • Interleukin17 antibody
    • Interleukin17A antibody
    • OTTHUMP00000016597 antibody
    • OTTMUSP00000046003 antibody
    see all

References

This product has been referenced in:
  • Liu L  et al. Periductal Mastitis: An Inflammatory Disease Related to Bacterial Infection and Consequent Immune Responses? Mediators Inflamm 2017:5309081 (2017). Read more (PubMed: 28182101) »
  • Kaewkangsadan V  et al. Crucial Contributions by T Lymphocytes (Effector, Regulatory, and Checkpoint Inhibitor) and Cytokines (TH1, TH2, and TH17) to a Pathological Complete Response Induced by Neoadjuvant Chemotherapy in Women with Breast Cancer. J Immunol Res 2016:4757405 (2016). IHC-P ; Human . Read more (PubMed: 27777963) »

See all 7 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Small bowel)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC1 (EDTA pH8.5)
Permeabilization
No
Specification
Small bowel
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 06 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Goat Serum as blocking agent for 30 minute(s) · Concentration: 50% · Temperature: 23°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer (pH 6.0)
Sample
Human Tissue sections (Colonic Mucosa)
Specification
Colonic Mucosa
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 02 2013

Thank you for your enquiry regarding ab9565.

We do not have any recommendations for lysis buffers, as we do not test this antibody on cell lysate in our labs. As the datasheet indicates, this antibody has been tested on recombinant hIL-17 at ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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