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Highly pure (>98%) recombinant hIL-17 (human Interleukin-17)
Our Abpromise guarantee covers the use of ab9565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|ELISA||Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-17 (assuming 100 µl/well antibody solution).|
|Neutralising||Use a concentration of 0.1 - 0.18 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hIL-17 (6.0 ng/ml).|
|IHC-P||Use at an assay dependent concentration.|
ab9565 staining IL17 in Human colonic mucosa tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 50% serum for 30 minutes at 23°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 20 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"