The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes ELISA: Use at a concentration of 0.15 - 0.30 µg/ml. This allows the detection of at least 0.2 ng/well of recombinant hIL17.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant hIL17 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 16 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionLigand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (PubMed:8676080).
Tissue specificityRestricted to activated memory T-cells.
Sequence similaritiesBelongs to the IL-17 family.
Post-translational modificationsFound both in glycosylated and nonglycosylated forms.