Anti-IL17A antibody [3.41.2.6.1] (Biotin) (ab155575)

Overview

  • Product name
    Anti-IL17A antibody [3.41.2.6.1] (Biotin)
    See all IL17A primary antibodies
  • Description
    Mouse monoclonal [3.41.2.6.1] to IL17A (Biotin)
  • Host species
    Mouse
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human IL17A protein (NM_002190.2).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 95% PBS, 4% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    3.41.2.6.1
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.125 - 0.25 µg/ml.

Target

  • Function
    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificity
    Restricted to activated memory T-cells.
  • Sequence similarities
    Belongs to the IL-17 family.
  • Post-translational
    modifications
    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CTLA 8 antibody
    • CTLA-8 antibody
    • CTLA8 antibody
    • cytotoxic T lymphocyte associated antigen 8 antibody
    • Cytotoxic T lymphocyte associated protein 8 antibody
    • Cytotoxic T lymphocyte associated serine esterase 8 antibody
    • Cytotoxic T-lymphocyte-associated antigen 8 antibody
    • IL 17 antibody
    • IL 17A antibody
    • IL-17 antibody
    • IL-17A antibody
    • IL17 antibody
    • IL17_HUMAN antibody
    • Il17a antibody
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) antibody
    • interleukin 17A antibody
    • Interleukin-17A antibody
    • OTTHUMP00000016597 antibody
    see all

Images

  • Sandwich ELISA analysis of Human IL17A using ab155575.

    Sandwich ELISA analysis of Human IL-17A monoclonal antibody was performed by coating a blank 96-well microtiter plate with 100µl per well of Human IL-17A moncolonal antibody in duplicate at 1, 3, 4, 5, 7, and 9µg/ml in DPBS and incubating for 12-18 hours at 4C. The plate was aspirated and blocked with 300µl per well of 4% BSA and 5% sucrose in DPBS for 1 hour at room temperature. Human IL-17A recombinant protein at 50µl per well was added in duplicate at 2000, 1000, 500, 250, 125, 62.5, 31.25, and 0 pg/ml for 2 hours at room temperature along with 50µl of Human IL-17A biotinylated monoclonal antibody (ab15575) in all applicable wells at 0.1µg/ml for 2 hours at room temperature. The plate was washed with ELISA Wash Buffer  and incubated with 100µl per well of Streptavidin-HRP in all test wells at 1:40,000 dilution for 30 minutes at room temperature and then washed and incubated with 100µl per well of TMB substrate for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.

References

ab155575 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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