Overview

  • Product name
  • Description
    Rabbit polyclonal to IL17C
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human IL17C.

    (Peptide available as ab94887.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: THP1; Raji; K562.

Properties

Applications

Our Abpromise guarantee covers the use of ab77183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1.
    • Sequence similarities
      Belongs to the IL-17 family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • CX2 antibody
      • Cytokine CX2 antibody
      • IL 17C antibody
      • IL 21 antibody
      • IL-17C antibody
      • IL17C antibody
      • IL17C_HUMAN antibody
      • Interleukin 17C antibody
      • Interleukin-17C antibody
      • MGC126884 antibody
      • MGC138401 antibody
      see all

    Images

    • All lanes : Anti-IL17C antibody (ab77183) at 1 µg/ml

      Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
      Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 21 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 20 minutes

    References

    ab77183 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab77183.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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