Overview

  • Product nameAnti-IL17D antibody
    See all IL17D primary antibodies
  • Description
    Rabbit polyclonal to IL17D
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human IL17D.

    (Peptide available as ab90991.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Human Thymus Tissue; Jurkat Whole Cell

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionInduces expression of IL-6, IL-8, and GM-CSF from endothelial cells.
  • Tissue specificityExpressed preferentially in adipose, skeletal muscle and CNS.
  • Sequence similaritiesBelongs to the IL-17 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IL 17D antibody
    • IL 22 antibody
    • IL 27 antibody
    • IL-17D antibody
    • IL-27 antibody
    • IL17D antibody
    • IL17D_HUMAN antibody
    • IL27 antibody
    • Interleukin 17D antibody
    • Interleukin 27 antibody
    • Interleukin-17D antibody
    • Interleukin-27 antibody
    see all

Anti-IL17D antibody images

  • All lanes : Anti-IL17D antibody (ab77185) at 1 µg/ml

    Lane 1 : Human thymus tissue lysate - total protein (ab30146)
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of 77185 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77185, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-IL17D antibody (ab77185)

ab77185 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"