Overview

  • Product nameAnti-IL17F antibody
    See all IL17F primary antibodies
  • Description
    Rabbit polyclonal to IL17F
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human IL17F aa 31-163. highly pure (>98%) recombinant hIL-17F (human IL-17F)
    Sequence:

    MRKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS MSRNIESRST SPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN SVPIQQETLV VRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ


    Database link: Q96PD4

Properties

Applications

Our Abpromise guarantee covers the use of ab46000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
ELISA Use a concentration of 0.5 - 2 µg/ml.

To detect IL17F by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab46001 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL17F.

WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 18 kDa.

Target

  • FunctionLigand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
  • Tissue specificityExpressed in activated, but not resting, CD4+ T-cells and activated monocytes.
  • Involvement in diseaseCandidiasis, familial, 6
  • Sequence similaritiesBelongs to the IL-17 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CANDF6 antibody
    • Cytokine ML 1 antibody
    • Cytokine ML-1 antibody
    • IL 17F antibody
    • IL 24 antibody
    • IL-17F antibody
    • Il17f antibody
    • IL17F_HUMAN antibody
    • Interleukin 17F antibody
    • Interleukin 24 antibody
    • Interleukin-17F antibody
    • ML 1 antibody
    • ML1 antibody
    • Mutant IL 17F antibody
    • OTTHUMP00000016602 antibody
    see all

Anti-IL17F antibody images

  • ab46000 (1µg/ml) staining IL17F in human ovary using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm of the corpus luteum.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • To detect IL17F by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab46001 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL17F.

References for Anti-IL17F antibody (ab46000)

ab46000 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"