Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab95340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15, 17 kDa (predicted molecular weight: 18 kDa).

Target

  • FunctionLigand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
  • Tissue specificityExpressed in activated, but not resting, CD4+ T-cells and activated monocytes.
  • Involvement in diseaseCandidiasis, familial, 6
  • Sequence similaritiesBelongs to the IL-17 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CANDF6 antibody
    • Cytokine ML 1 antibody
    • Cytokine ML-1 antibody
    • IL 17F antibody
    • IL 24 antibody
    • IL-17F antibody
    • Il17f antibody
    • IL17F_HUMAN antibody
    • Interleukin 17F antibody
    • Interleukin 24 antibody
    • Interleukin-17F antibody
    • ML 1 antibody
    • ML1 antibody
    • Mutant IL 17F antibody
    • OTTHUMP00000016602 antibody
    see all

Anti-IL17F antibody images

  • All lanes : Anti-IL17F antibody (ab95340) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
    Lane 3 : LPS-Treated HL60 Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 15,17 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,150 kDa,60 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes

References for Anti-IL17F antibody (ab95340)

ab95340 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab95340.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"