Overview

  • Product name
  • Description
    Rabbit polyclonal to IL17RD
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 286 - 501 of Human IL17RD (NP_001074442).

  • Positive control
    • 293T, A431 , H1299, HeLa S3 and NIH3T3 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab96294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 82 kDa.

Target

  • Function
    Feedback inhibitor of fibroblast growth factor mediated Ras-MAPK signaling and ERK activation. May inhibit FGF-induced FGFR1 tyrosine phosphorylation. Regulates the nuclear ERK signaling pathway by spatially blocking nuclear translocation of activated ERK without inhibiting cytoplasmic phosphorylation of ERK. Mediates JNK activation and may be involved in apoptosis.
  • Tissue specificity
    Expressed in umbilical vein endothelial cells and in several highly vascularized tissues such as kidney, colon, skeletal muscle, heart and small intestine. Highly expressed in ductal epithelial cells of salivary glands, seminal vesicles and the collecting tubules of the kidney. Isoform 1 is also highly expressed in both fetal and adult brain, pituarity, tonsils, spleen, adenoids, fetal kidney, liver, testes and ovary. Isoform 1 is also expressed at moderate levels in primary aortic endothelial cells and adrenal medulla, and at low levels in adrenal cortex. Isoform 4 is specifically and highly expressed in pituitary, fetal brain and umbilical vein endothelial cells.
  • Cellular localization
    Cytoplasm and Golgi apparatus membrane. Cell membrane. Predominantly associated with the Golgi apparatus and is partially translocated to the plasma membrane upon stimulation.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434N1928 antibody
    • FLJ35755 antibody
    • hSef antibody
    • I17RD_HUMAN antibody
    • IL 17 receptor D antibody
    • IL 17RD antibody
    • IL-17 receptor D antibody
    • IL-17RD antibody
    • IL17 receptor D antibody
    • il17rd antibody
    • IL17Rhom antibody
    • IL17RLM antibody
    • Interleukin 17 receptor D antibody
    • Interleukin 17 receptor like protein antibody
    • Interleukin-17 receptor D antibody
    • Interleukin-17 receptor-like protein antibody
    • Interleukin17 receptor D antibody
    • MGC133309 antibody
    • SEF antibody
    • Sef homolog antibody
    see all

Anti-IL17RD antibody images

  • Anti-IL17RD antibody (ab96294) at 1/2000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size : 82 kDa
  • Anti-IL17RD antibody (ab96294) at 1/500 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 82 kDa

References for Anti-IL17RD antibody (ab96294)

This product has been referenced in:
  • Iwamoto N  et al. Context-specific flow through the MEK/ERK module produces cell- and ligand-specific patterns of ERK single and double phosphorylation. Sci Signal 9:ra13 (2016). Read more (PubMed: 26838549) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U373)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
50 µg
Specification
U373
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Apr 13 2017

The main difference between the 2 antibodies you have selected, ab96294 and ab111553, is the immunogen. ab96294 was raised against an immunogen within amino acids 286-501 of human IL17RD, which represent cytoplasmic residues of the receptor. ab111553 w...

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Thank you for contacting us.
Either antibody should work well for human cells in WB, the only different is their immunogen and a slightly different concentration. If I had to choose, I think I would go with ab111553 because it is supllied at a slig...

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