Overview

  • Product nameAnti-IL17RE antibody
  • Description
    Rabbit polyclonal to IL17RE
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human IL17RE.

    (Peptide available as ab93797.)

  • Positive control
    • This antibody gave a positive signal in Jurkat whole cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/250. Detects a band of approximately 71 kDa (predicted molecular weight: 74 kDa).

Target

  • RelevanceReceptor which can activate the MAPK signaling pathway and seems to function at, or upstream of Ras. Isoform 2 and isoform 4 may be either cytoplasmic inactive or dominant active forms. Isoform 3 and isoform 5 may act as soluble decoy receptors
  • Cellular localizationMembrane (isoform 1), Cytoplasm (isoforms 2 + 4), Secreted (isoforms 3 + 5)
  • Database links
  • Alternative names
    • FLJ23658 antibody
    • IL-17 receptor E antibody
    • IL-17RE antibody
    • interleukin 17 receptor E antibody
    • Interleukin-17 receptor E antibody
    • MGC71884 antibody
    • OTTHUMP00000158822 antibody
    • UNQ3056/PRO9877 antibody
    see all

Anti-IL17RE antibody images

  • Anti-IL17RE antibody (ab77527) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 74 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • IHC image of IL17RE staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77527, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-IL17RE antibody (ab77527)

ab77527 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"