Overview

  • Product nameAnti-IL18 antibody
    See all IL18 primary antibodies
  • Description
    Rabbit polyclonal to IL18
  • SpecificityImmunogen seqeuence suggest that the product will recognise both the full length and mature form of the protein but this has not been tested specifically.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human IL18.

    (Peptide available as ab101221.)

  • Positive control
    • This antibody gave a positive signal in the following human whole cell lysates: HL60; Jurkat. IHC-P: FFPE human tonsil tissue sections.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab68435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Target

  • FunctionAugments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Iboctadekin antibody
    • IFN gamma inducing factor antibody
    • IFN-gamma-inducing factor antibody
    • IGIF antibody
    • IL 1 gamma antibody
    • IL 18 antibody
    • IL 1g antibody
    • IL-1 gamma antibody
    • IL-18 antibody
    • IL1 gamma antibody
    • IL18 antibody
    • IL18 protein antibody
    • IL18_HUMAN antibody
    • IL1F4 antibody
    • IL1g antibody
    • IL1gamma antibody
    • ILIF4 antibody
    • Interferon gamma inducing factor antibody
    • Interferon gamma-inducing factor antibody
    • Interleukin 1 gamma antibody
    • Interleukin 18 (interferon-gamma-inducing factor) antibody
    • Interleukin 18 antibody
    • Interleukin-1 gamma antibody
    • Interleukin-18 antibody
    • Interleukin18 antibody
    • MGC12320 antibody
    see all

Anti-IL18 antibody images

  • IHC image of IL18 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68435, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-IL18 antibody (ab68435) at 1 µg/ml

    Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-IL18 antibody (ab68435)

ab68435 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Treatment 0.1 ug/ml LPS 15 min
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"