Overview

  • Product nameAnti-IL18 antibody
    See all IL18 primary antibodies
  • Description
    Rabbit polyclonal to IL18
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse IL18.

  • Positive control
    • WB: Mouse spleen tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab71495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IP Use a concentration of 2 - 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionAugments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Iboctadekin antibody
    • IFN gamma inducing factor antibody
    • IFN-gamma-inducing factor antibody
    • IGIF antibody
    • IL 1 gamma antibody
    • IL 18 antibody
    • IL 1g antibody
    • IL-1 gamma antibody
    • IL-18 antibody
    • IL1 gamma antibody
    • IL18 antibody
    • IL18 protein antibody
    • IL18_HUMAN antibody
    • IL1F4 antibody
    • IL1g antibody
    • IL1gamma antibody
    • ILIF4 antibody
    • Interferon gamma inducing factor antibody
    • Interferon gamma-inducing factor antibody
    • Interleukin 1 gamma antibody
    • Interleukin 18 (interferon-gamma-inducing factor) antibody
    • Interleukin 18 antibody
    • Interleukin-1 gamma antibody
    • Interleukin-18 antibody
    • Interleukin18 antibody
    • MGC12320 antibody
    see all

Anti-IL18 antibody images

  • Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded murine obsese nephric tissue sections, labelling IL18 with ab71495 at a dilution of 1/2500 incubated for 12 hours at 4°C in PBS and 2.5% horse serum diluent. Heat mediated antigen retrival was with citrate buffer and blocking was with 2.5% horse serum in PBS incubated for 1 hour at 25°C. Secondary was a horse anti-rabbit polyclonal HRP conjugate.

    See Abreview

  • Anti-IL18 antibody (ab71495) at 1 µg/ml + Mouse spleen tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 100 kDa,150 kDa,76 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • IHC image of ab71495 staining in mouse normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71495, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-IL18 antibody (ab71495)

This product has been referenced in:
  • Cao S  et al. Hydrogen sulfide attenuates brain edema in early brain injury after subarachnoid hemorrhage in rats: Possible involvement of MMP-9 induced blood-brain barrier disruption and AQP4 expression. Neurosci Lett 621:88-97 (2016). WB ; Rat . Read more (PubMed: 27080433) »
  • Qiao J  et al. Busulfan and cyclosphamide induce liver inflammation through NLRP3 activation in mice after hematopoietic stem cell transplantation. Sci Rep 5:17828 (2015). Mouse . Read more (PubMed: 26635145) »

See all 5 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer 1X
Permeabilization No
Specification kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 14 2015

Thank you very much for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a fresh vial of ab71495 on the order ***, which should arrive tomorrow.

Please keep me updated about ...

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ab71495 will detect both the forms of IL-18.

Hope this information has been helpful for you.Please let me know if you have any other questions.

Thank you for contacting us and for your interest in our products.

I am sorry to confirm that as far as we are aware, ab71495 has never been tested with samples from dog. All tested species cross-reactivity information covered by our guarant...

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Thank you for your enquiry. The epitope region for this product has not been mapped, therefore we do not have the exact epitope information. Unfortunately, the exact peptide sequence information is also not available (proprietary and can not be rel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"