Recombinant
RabMAb

Anti-IL18 binding protein antibody [EP1088Y] (ab52914)

Overview

  • Product name
    Anti-IL18 binding protein antibody [EP1088Y]
    See all IL18 binding protein primary antibodies
  • Description
    Rabbit monoclonal [EP1088Y] to IL18 binding protein
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human IL18 binding protein aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O95998

  • Positive control
    • WB: HeLa and K562 cell lysates and rat kidney and heart tissue lysates. IHC-P: human tonsil tissue.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
IHC-P 1/400.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
    • Tissue specificity
      Strongly expressed in heart, lung, placenta and spleen.
    • Sequence similarities
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • I18BP_HUMAN antibody
      • IL-18BP antibody
      • IL18 BP antibody
      • IL18 BPa antibody
      • IL18BP antibody
      • IL18BPa antibody
      • Interleukin 18 binding protein antibody
      • Interleukin-18-binding protein antibody
      • MC51L 53L 54L homolog gene product antibody
      • Tadekinig alfa antibody
      • Tadekinig-alfa antibody
      see all

    Images

    • All lanes : Anti-IL18 binding protein antibody [EP1088Y] (ab52914) at 1/2000 dilution (purified)

      Lane 1 : Rat kidney tissue lysate
      Lane 2 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 22 kDa
      Observed band size : 44 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 1% BSA/TBST.

    • All lanes : Anti-IL18 binding protein antibody [EP1088Y] (ab52914) at 1/1000 dilution (purified)

      Lane 1 : HeLa whole cell lysate
      Lane 2 : K562 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 22 kDa
      Observed band size : 44 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 1% BSA/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IL18 binding protein with purified ab52914 at 1/400. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Anti-IL18 binding protein antibody [EP1088Y] (ab52914) at 1/500 dilution (unpurified) + HeLa cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 22 kDa
      Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IL18 binding protein with unpurified ab52914 at a dilution of 1/50.

    References

    This product has been referenced in:
    • Lin L  et al. Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma. Sci Rep 6:26757 (2016). IHC . Read more (PubMed: 27225603) »
    • Carbotti G  et al. The IL-18 antagonist IL-18-binding protein is produced in the human ovarian cancer microenvironment. Clin Cancer Res 19:4611-20 (2013). IHC ; Human . Read more (PubMed: 23873689) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (gel 12 %)
    Sample
    Rat Cell lysate - whole cell (Kidney)
    Specification
    Kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 29 2014

    Thank you for contacting us.

    The recommended protocol can be found at the following link;

    http://www.epitomics.com/products/support/fc

    The antibody is guaranteed for Flow Cytometry and it would work. if it does not we will r...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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