Anti-IL18 binding protein antibody [EP1088Y] (ab52914)

Overview

  • Product nameAnti-IL18 binding protein antibody [EP1088Y]
    See all IL18 binding protein primary antibodies
  • Description
    Rabbit monoclonal [EP1088Y] to IL18 binding protein
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human IL18 binding protein aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O95998

  • Positive control
    • WB: HeLa and K562 cell lysates and rat kidney and heart tissue lysates. IHC-P: human tonsil tissue.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
IHC-P 1/400.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • Application notesIs unsuitable for Flow Cyt or ICC/IF.
  • Target

    • FunctionIsoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
    • Tissue specificityStrongly expressed in heart, lung, placenta and spleen.
    • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • I18BP_HUMAN antibody
      • IL-18BP antibody
      • IL18 BP antibody
      • IL18 BPa antibody
      • IL18BP antibody
      • IL18BPa antibody
      • Interleukin 18 binding protein antibody
      • Interleukin-18-binding protein antibody
      • MC51L 53L 54L homolog gene product antibody
      • Tadekinig alfa antibody
      • Tadekinig-alfa antibody
      see all

    Anti-IL18 binding protein antibody [EP1088Y] images

    • All lanes : Anti-IL18 binding protein antibody [EP1088Y] (ab52914) at 1/2000 dilution (purified)

      Lane 1 : Rat kidney tissue lysate
      Lane 2 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 22 kDa
      Observed band size : 44 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 1% BSA/TBST.

    • All lanes : Anti-IL18 binding protein antibody [EP1088Y] (ab52914) at 1/1000 dilution (purified)

      Lane 1 : HeLa whole cell lysate
      Lane 2 : K562 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 22 kDa
      Observed band size : 44 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 1% BSA/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IL18 binding protein with purified ab52914 at 1/400. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Anti-IL18 binding protein antibody [EP1088Y] (ab52914) at 1/500 dilution (unpurified) + HeLa cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 22 kDa
      Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IL18 binding protein with unpurified ab52914 at a dilution of 1/50.

    References for Anti-IL18 binding protein antibody [EP1088Y] (ab52914)

    This product has been referenced in:
    • Carbotti G  et al. The IL-18 antagonist IL-18-binding protein is produced in the human ovarian cancer microenvironment. Clin Cancer Res 19:4611-20 (2013). IHC ; Human . Read more (PubMed: 23873689) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 15 µg
    Gel Running Conditions Reduced Denaturing (gel 12 %)
    Sample Rat Cell lysate - whole cell (Kidney)
    Specification Kidney
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 29 2014

    Thank you for contacting us.

    The recommended protocol can be found at the following link;

    http://www.epitomics.com/products/support/fc

    The antibody is guaranteed for Flow Cytometry and it would work. if it does not we will r...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"