The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Use 10 µl to label 106 cells or 100 µl of whole blood.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionRequired for the high affinity binding of interleukin 18 (IL-18) to its receptor complex (By similarity). Together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK.
Tissue specificityStrongly expressed in peripheral blood leukocytes, spleen, lung, and, to a lesser extent, in colon, but not in any other tissue tested. Strongly expressed in T-cells polarized with IL-12. Specifically coexpressed with IL18R1 in Th1 cells.
Sequence similaritiesBelongs to the interleukin-1 receptor family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 TIR domain.