Overview

  • Product nameAnti-IL1RA antibodySee all IL1RA primary antibodies ...
  • Description
    Rabbit polyclonal to IL1RA
  • SpecificityThis antibody does not recognize human IL-1 alpha , IL-1 beta or IL-1 receptor type I or type II. It is primarily directed against the secreted form of IL-1Ra (also termed sIL-1Ra) but recognizes the native (non-denatured) as well as denatured intracellular form of IL-1Ra (also termed icIL-1Ra).
  • Tested applicationsICC/IF, ELISA, Flow Cyt, IHC-P, IHC-Fr, Neutralising, RIA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non Human Primates

    Does not react with

    Mouse, Rabbit
  • Immunogen

    Recombinant human IL-1Ra produced in E.coli.

  • Positive control

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesSterile filtered, no stabilizer. Endotoxin Content: <10 pg/ml by LAL method.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab2573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/500 - 1/1000.
Flow Cyt Use at an assay dependent dilution. Caution should be exhibited as the F(c) domain of the rabbit IgG molecule may non-specifically interact with cells.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Neutralising Use at an assay dependent dilution. See protocol page.
RIA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 17 kDa (predicted molecular weight: 20 kDa).

Target

  • FunctionInhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
  • Tissue specificityThe intracellular form of IL1RN is predominantly expressed in epithelial cells.
  • Involvement in diseaseGenetic variation in IL1RN is associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4) [MIM:612628]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
    Defects in IL1RN are the cause of interleukin 1 receptor antagonist deficiency (DIRA) [MIM:612852]; also known as deficiency of interleukin 1 receptor antagonist. Autoinflammatory diseases manifest inflammation without evidence of infection, high-titer autoantibodies, or autoreactive T-cells. DIRA is a rare, autosomal recessive, genetic autoinflammatory disease that results in sterile multifocal osteomyelitis (bone inflammation in multiple places), periostitis (inflammation of the membrane surrounding the bones), and pustulosis (due to skin inflammation) from birth.
  • Cellular localizationCytoplasm and Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • DIRA antibody
    • ICIL 1RA antibody
    • ICIL-1RA antibody
    • ICIL1RA antibody
    • IL-1ra antibody
    • IL-1ra3 antibody
    • IL-1RN antibody
    • IL1 inhibitor antibody
    • IL1F3 antibody
    • IL1RA antibody
    • IL1RA_HUMAN antibody
    • IL1RN (IL1F3) antibody
    • IL1RN antibody
    • Interleukin 1 receptor antagonist antibody
    • Interleukin-1 receptor antagonist protein antibody
    • Intracellular IL 1 receptor antagonist type II antibody
    • Intracellular interleukin 1 receptor antagonist (icIL 1ra) antibody
    • IRAP antibody
    • MGC10430 antibody
    • MVCD4 antibody
    • Type II interleukin 1 receptor antagonist antibody
    see all

Anti-IL1RA antibody images

  • ICC/IF image of ab2573 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2573, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Anti-IL1RA antibody (ab2573) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa


    Exposure time : 3 minutes
  • Anti-IL1RA antibody (ab2573) at 1 µg/ml + Human IL1RA full length protein (ab50081) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds

References for Anti-IL1RA antibody (ab2573)

This product has been referenced in:
  • Tjomsland V  et al. Interleukin 1a sustains the expression of inflammatory factors in human pancreatic cancer microenvironment by targeting cancer-associated fibroblasts. Neoplasia 13:664-75 (2011). IHC-P ; Human . Read more (PubMed: 21847358) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human mesenchymal stem cells)
Specification Human mesenchymal stem cells
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Mr. Hao Wu

Verified customer

Submitted Dec 03 2010

Application Western blot
Sample Human Tissue lysate - whole (PBMC)
Loading amount 25 µg
Specification PBMC
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 12 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"