The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects bands of approximately 20 and 40 kDa (predicted molecular weight: 20 kDa). We have no information regarding the 40 kDa band.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
The intracellular form of IL1RN is predominantly expressed in epithelial cells.
Involvement in disease
Microvascular complications of diabetes 4 Interleukin 1 receptor antagonist deficiency