Anti-IL1RA antibody [AS17], prediluted (FITC) (ab28315)

Overview

  • Product nameAnti-IL1RA antibody [AS17], prediluted (FITC)See all IL1RA primary antibodies ...
  • Description
    Mouse monoclonal [AS17] to IL1RA, prediluted (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab28315 recognizes human IL1RA. Does not recognize hIL1a, hIL1b or a panel of other human cytokines.
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • Whole blood

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesProtein G purified from in vitro cell culture. Supplied as a 0.2µm sterile filtered solution. This antibody is > 95 % pure, by SDS PAGE analysis.
  • Clonality Monoclonal
  • Clone numberAS17
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab28315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 10µl for 106 cells. Mix gently and incubate for 15 minutes at 2°C to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
    Use 10µl for 100µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
    • Tissue specificityThe intracellular form of IL1RN is predominantly expressed in epithelial cells.
    • Involvement in diseaseGenetic variation in IL1RN is associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4) [MIM:612628]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
      Defects in IL1RN are the cause of interleukin 1 receptor antagonist deficiency (DIRA) [MIM:612852]; also known as deficiency of interleukin 1 receptor antagonist. Autoinflammatory diseases manifest inflammation without evidence of infection, high-titer autoantibodies, or autoreactive T-cells. DIRA is a rare, autosomal recessive, genetic autoinflammatory disease that results in sterile multifocal osteomyelitis (bone inflammation in multiple places), periostitis (inflammation of the membrane surrounding the bones), and pustulosis (due to skin inflammation) from birth.
    • Cellular localizationCytoplasm and Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • DIRA antibody
      • ICIL 1RA antibody
      • ICIL-1RA antibody
      • ICIL1RA antibody
      • IL-1ra antibody
      • IL-1ra3 antibody
      • IL-1RN antibody
      • IL1 inhibitor antibody
      • IL1F3 antibody
      • IL1RA antibody
      • IL1RA_HUMAN antibody
      • IL1RN (IL1F3) antibody
      • IL1RN antibody
      • Interleukin 1 receptor antagonist antibody
      • Interleukin-1 receptor antagonist protein antibody
      • Intracellular IL 1 receptor antagonist type II antibody
      • Intracellular interleukin 1 receptor antagonist (icIL 1ra) antibody
      • IRAP antibody
      • MGC10430 antibody
      • MVCD4 antibody
      • Type II interleukin 1 receptor antagonist antibody
      see all

    Anti-IL1RA antibody [AS17], prediluted (FITC) images

    • Overlay histogram showing peripheral blood lymphocytes cells stained with ab28315 (red line). The cells were fixed with 4% paraformaldehydel (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28315, 0.25µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (0.25µg/1x106 cells ). Acquisition of >5,000 events was performed.

    References for Anti-IL1RA antibody [AS17], prediluted (FITC) (ab28315)

    ab28315 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab28315.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"