Anti-IL1RA antibody [AS17], prediluted (FITC) (ab28315)

Overview

  • Product name
    Anti-IL1RA antibody [AS17], prediluted (FITC)
    See all IL1RA primary antibodies
  • Description
    Mouse monoclonal [AS17] to IL1RA, prediluted (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    ab28315 recognizes human IL1RA. Does not recognize hIL1a, hIL1b or a panel of other human cytokines.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • Whole blood

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Protein G purified from in vitro cell culture. Supplied as a 0.2µm sterile filtered solution. This antibody is > 95 % pure, by SDS PAGE analysis.
  • Clonality
    Monoclonal
  • Clone number
    AS17
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab28315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 10µl for 106 cells. Mix gently and incubate for 15 minutes at 2°C to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
    Use 10µl for 100µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
    • Tissue specificity
      The intracellular form of IL1RN is predominantly expressed in epithelial cells.
    • Involvement in disease
      Microvascular complications of diabetes 4
      Interleukin 1 receptor antagonist deficiency
    • Cellular localization
      Cytoplasm and Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • DIRA antibody
      • F630041P17Rik antibody
      • ICIL 1RA antibody
      • ICIL-1RA antibody
      • ICIL1RA antibody
      • IL-1ra antibody
      • IL-1ra3 antibody
      • IL-1RN antibody
      • IL1 inhibitor antibody
      • IL1F3 antibody
      • IL1RA antibody
      • IL1RA_HUMAN antibody
      • IL1RN (IL1F3) antibody
      • IL1RN antibody
      • Interleukin 1 receptor antagonist antibody
      • Interleukin-1 receptor antagonist protein antibody
      • Intracellular IL 1 receptor antagonist type II antibody
      • Intracellular interleukin 1 receptor antagonist (icIL 1ra) antibody
      • IRAP antibody
      • MGC10430 antibody
      • MVCD4 antibody
      • Type II interleukin 1 receptor antagonist antibody
      see all

    Images

    • Overlay histogram showing peripheral blood lymphocytes cells stained with ab28315 (red line). The cells were fixed with 4% paraformaldehydel (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28315, 0.25µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (0.25µg/1x106 cells ). Acquisition of >5,000 events was performed.

    References

    ab28315 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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