The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Stable for one year.
The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAP). IL-1RAP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor associated kinase (IRAK), which mediates activation of NF-kB inducing kinae (NIK) and of NF-kB, recruits to the IL-1Rcomplex through IL-1RAP. IL-1 activation of stress activated protein kinase and of acid sphingomyelinase also requires IL-1RAP. Like IL-1RI, IL-1RAP subunit is essential for IL-1 mediated cellular response. IL-1RAP is expressed in many tissues.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
FunctionCoreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
Tissue specificityDetected in liver, skin, placenta, thymus and lung.
Sequence similaritiesBelongs to the interleukin-1 receptor family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 TIR domain.