The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at a concentration of 1 µg/ml to detect 0.6 ng/well of recombinant human IL2.
Neut: Use at a concentration of 0.8 - 8.0 µg/ml.
WB: Use at a concentration of 2 µg/ml, this detects 5 ng/lane of recombinant human IL2 under reducing conditions and 20 ng/lane under non-reducing conditions.Predicted molecular weight: 19 kDa.
Anti-Human IL2 is tested for its ability to neutralize the biological activity of recombinant, human IL2 on CTLL2 cells. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of recombinant, human IL2, when IL2 is present at a concentration just high enough to elicit a maximum response.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionProduced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Involvement in diseaseNote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.