Anti-IL-2 antibody [AP-MAB0844] (ab80681)
Key features and details
- Rat monoclonal [AP-MAB0844] to IL-2
- Suitable for: IHC-Fr, IP, Flow Cyt, Neutralising
- Reacts with: Mouse
- Isotype: IgG2
Overview
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Product name
Anti-IL-2 antibody [AP-MAB0844]
See all IL-2 primary antibodies -
Description
Rat monoclonal [AP-MAB0844] to IL-2 -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, IP, Flow Cyt, Neutralisingmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein corresponding to Mouse IL-2.
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General notes
Endotoxin Level: Extremely low level of LPS (< 0.002EU/µg IgG).ab80681 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
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Properties
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Form
Lyophilized:Reconstitute with 1 ml sterile PBS. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
The IgG fraction of culture supernatant was purified by Protein A affinity chromatography. -
Primary antibody notes
ab80681 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat). -
Clonality
Monoclonal -
Clone number
AP-MAB0844 -
Isotype
IgG2 -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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Neutralising |
Use at an assay dependent concentration.
ab80681 can be used for neutralizing mouse IL2 in vitro. |
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
Neutralising
Use at an assay dependent concentration. ab80681 can be used for neutralizing mouse IL2 in vitro. |
Target
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Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 16183 Mouse
- SwissProt: P04351 Mouse
- Unigene: 14190 Mouse
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Alternative names
- Aldesleukin antibody
- IL 2 antibody
- IL-2 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab80681 has been referenced in 1 publication.
- Borghesi J et al. Canine amniotic membrane mesenchymal stromal/stem cells: Isolation, characterization and differentiation. Tissue Cell 58:99-106 (2019). PubMed: 31133253