Recombinant Anti-IL-2 antibody [EPR2780] (ab92381)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2780] to IL-2
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-2 antibody [EPR2780]
See all IL-2 primary antibodies -
Description
Rabbit monoclonal [EPR2780] to IL-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt (Intra),IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human IL-2 aa 50-150. The exact sequence is proprietary.
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Positive control
- WB: Recombinant human IL-2 full length protein and Jurkat (Human T cell leukemia cell line from peripheral blood) treated with 40nM Phorbol-12-myristate-13-acetate, 2 µM A23187 and 300 ng/ml Brefeldin A (45 min delay) overnight whole cell lysate. ICC/IF: PBMC cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2780 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3558 Human
- Omim: 147680 Human
- SwissProt: P60568 Human
- Unigene: 89679 Human
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Alternative names
- Aldesleukin antibody
- IL 2 antibody
- IL-2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence analysis of PBMC (Human primary peripheral blood mononuclear cell) cells labeling IL-2 using ab92381. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab92381 at 1:50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.
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Anti-IL-2 antibody [EPR2780] (ab92381) at 1/1000 dilution + Recombinant human IL-2 full length protein, 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-IL-2 antibody [EPR2780] (ab92381) at 1/5000 dilution
Lane 1 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) treated with 40nM Phorbol-12-myristate-13-acetate, 2 µM A23187 and 300 ng/ml Brefeldin A (45 min delay) overnight whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab207325 is recommended to detect endogenous IL-2.
ab181602 was used as GAPDH loading control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab92381 has been referenced in 17 publications.
- Šerstņova K et al. Expression of anti-inflammatory markers IL-2, IL-10, TGF-β1, βDEF-2, βDEF-3 and Cathelicidin LL37 in dairy cattle milk with different health status of the udder. Pol J Vet Sci 25:237-248 (2022). PubMed: 35861957
- Wang Q et al. Morphine suppresses the immune function of lung cancer by up-regulating MAEL expression. BMC Pharmacol Toxicol 23:92 (2022). PubMed: 36476246
- Reiser SC et al. Profiling and Characterization of Localized Cytokine Response in Congenital Cleft Affected Lip Tissue. Life (Basel) 11:N/A (2021). PubMed: 34199238
- Hashimoto K et al. Characterization of PD-1/PD-L1 immune checkpoint expression in soft tissue sarcomas. Eur J Histochem 65:N/A (2021). PubMed: 34218652
- Huang X et al. Alterations in CD8+ Tregs, CD56+ Natural Killer Cells and IL-10 Are Associated With Invasiveness of Nonfunctioning Pituitary Adenomas (NFPAs). Pathol Oncol Res 27:598887 (2021). PubMed: 34257554