Overview

  • Product name
    Anti-IL2 antibody [EPR2780]
    See all IL2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2780] to IL2
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human IL2.

  • Positive control
    • Human thymus and PBMC cell lysates, Human tonsil tissue; Jurkat cell lydate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-IL2 antibody (Alexa Fluor® 647) [EPR2780] (ab199215)

    Anti-CD99 antibody (Alexa Fluor® 488) [EPR3096] (ab208372)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR2780
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
Flow Cyt 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
    • Involvement in disease
      Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
    • Sequence similarities
      Belongs to the IL-2 family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Aldesleukin antibody
      • IL 2 antibody
      • IL-2 antibody
      • IL2 antibody
      • IL2_HUMAN antibody
      • Interleukin 2 antibody
      • Interleukin-2 antibody
      • Involved in regulation of T cell clonal expansion antibody
      • Lymphokine antibody
      • OTTHUMP00000164090 antibody
      • POIL2 antibody
      • T Cell Growth Factor antibody
      • T-cell growth factor antibody
      • TCGF antibody
      see all

    Images

    • All lanes : Anti-IL2 antibody [EPR2780] (ab92381) at 1/1000 dilution

      Lane 1 : Human thymus lysate
      Lane 2 : PBMC lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 18 kDa
    • Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling IL2 with purified ab92381 at 1/100 dilution (10ug/mL) (red). Cells were fixed with 80% methanol and permeabilised using 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black) (ab172730). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • ab92381 at 1/250 dilution staining IL2 in Formalin-fixed, Paraffin-embedded Human tonsil tissue. Detection: DAB staining.

    References

    This product has been referenced in:
    • Kaewkangsadan V  et al. Crucial Contributions by T Lymphocytes (Effector, Regulatory, and Checkpoint Inhibitor) and Cytokines (TH1, TH2, and TH17) to a Pathological Complete Response Induced by Neoadjuvant Chemotherapy in Women with Breast Cancer. J Immunol Res 2016:4757405 (2016). IHC-P ; Human . Read more (PubMed: 27777963) »
    • Verma C  et al. Natural killer (NK) cell profiles in blood and tumour in women with large and locally advanced breast cancer (LLABC) and their contribution to a pathological complete response (PCR) in the tumour following neoadjuvant chemotherapy (NAC): differential restoration of blood profiles by NAC and surgery. J Transl Med 13:180 (2015). IHC . Read more (PubMed: 26040463) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab92381.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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