Human IL-2 ELISA Set (without plates) (ab48471)
Overview
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Product name
Human IL-2 ELISA Set (without plates)
See all IL-2 kits -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
1000 pg/ml - 31.25 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-2 Human in vitro ELISA Set is designed for the quantitative measurement of IL-2 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The IL-2 ELISA set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA set is intended to measure human IL-2 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-IL-2 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml IL-2 Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml IL-2 Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials Ready-to-Use TMB 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
Research areas
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Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Aldesleukin
- IL 2
- IL-2
see all -
Database links
- Entrez Gene: 3558 Human
- Omim: 147680 Human
- SwissProt: P60568 Human
- Unigene: 89679 Human
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab48471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use at an assay dependent concentration.
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Notes |
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Sandwich ELISA
Use at an assay dependent concentration. |
Datasheets and documents
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Datasheet download
References (0)
ab48471 has not yet been referenced specifically in any publications.