Overview

  • Product nameIL2 Human ELISA Set (without plates)
    See all IL2 kits
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    1000 pg/ml - 31.25 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-2 Human in vitro ELISA Set is designed for the quantitative measurement of IL-2 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

    The IL-2 ELISA set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

    This ELISA set is intended to measure human IL-2 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

  • Tested applicationsSandwich ELISAmore details
  • PlatformReagents

Properties

  • FunctionProduced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • Involvement in diseaseNote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • Sequence similaritiesBelongs to the IL-2 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin-2
    • lymphokine
    • T cell growth factor
    • T-cell growth factor
    • TCGF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab48471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

IL2 Human ELISA Set (without plates) images

  • IL-2 Standard curve ranging from 31.25 to 1000 pg/mL

References for IL2 Human ELISA Set (without plates) (ab48471)

ab48471 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"