The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.2µg for 106 cells. ab154436-Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
FunctionReceptor for interleukin-2.
Involvement in diseaseGenetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
References for Anti-IL2 Receptor alpha antibody [7D4] (Allophycocyanin) (ab25486)
This product has been referenced in:
Anraku M et al. Synergistic antitumor effects of regulatory T cell blockade combined with pemetrexed in murine malignant mesothelioma. J Immunol185:956-66 (2010).
Read more (PubMed: 20548032) »
Lorenzo F et al. Structure-function study of the p55 subunit of murine IL-2 receptor by epitope mapping. J Immunol147:2970-7 (1991).
Read more (PubMed: 1717581) »