The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.2µg for 106 cells. ab154451-Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
FunctionReceptor for interleukin-2.
Involvement in diseaseGenetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Ab24933, at a concentration of 0.1µg for 106 cells, staining CD25 in cells from BALB/c spleen that had been activated by PMA (10 ng/ml) and ionomycin (400 ng/ml) for 48 hours. Stained cells were then analyzed on a flow cytometer.
References for Anti-IL2 Receptor alpha antibody [7D4] (Phycoerythrin) (ab24933)
has not yet been referenced specifically in any publications.
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