Anti-IL2 Receptor alpha antibody [7D4] (Phycoerythrin) (ab24933)

Overview

• Product name
  Anti-IL2 Receptor alpha antibody [7D4] (Phycoerythrin)
  See all IL2 Receptor alpha primary antibodies
• Description
  Rat monoclonal [7D4] to IL2 Receptor alpha (Phycoerythrin)
• Conjugation
  Phycoerythrin. Ex: 488nm, Em: 575nm
• Specificity
  This antibody recognises low affinity IL2 receptor alpha.
• Tested applications
  Suitable for: Flow Cyt, IPmore details
• Species reactivity
  Reacts with: Mouse
  
• Immunogen

  Tissue/ cell preparation: IL2 dependent BALB/c mouse helper T cell clone HT2.

• Epitope
  This antibody reacts with an epitope located between amino acids 125 and 212 of IL2 receptor alpha.
• Positive control
  • Cells from BALB/c spleen activated with PMA and ionomycin for Immunofluorescent staining with Flow Cytometric analysis.
• General notes

  
  

  This antibody does not block the binding of IL2 to CD25.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C.
• Storage buffer
  Preservative: 0.09% Sodium Azide
  Constituents: 16% Sucrose, PBS; stabilizing agent
• Concentration information loading...
• Purity
  Purified IgM
• Primary antibody notes
  This antibody does not block the binding of IL2 to CD25.
• Clonality
  Monoclonal
• Clone number
  7D4
• Isotype
  IgM
• Light chain type
  kappa
• Research areas
  • Immunology
  • Adaptive Immunity
  • T Cells
  • CD

  • Immunology
  • Cell Type Markers
  • CD
  • Cytokines

  • Immunology
  • Innate Immunity
  • Cytokines
  • Interleukins

  • Immunology
  • Adaptive Immunity
  • Regulatory T Cells

Associated products

• Compatible Secondaries
  • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
  • Goat Anti-Rat IgG H&L (HRP) (ab205720)

Applications

Target

• Function
  Receptor for interleukin-2.
• Involvement in disease
  Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
• Sequence similarities
  Contains 2 Sushi (CCP/SCR) domains.
• Cellular localization
  Membrane.

• Target information above from: UniProt accession P01589 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 16184 Mouse
  • SwissProt: P01590 Mouse
  • Unigene: 915 Mouse
• Alternative names
  • Interleukin 2 receptor alpha chain antibody
  • CD25 antibody
  • CD25 antigen antibody

  • IDDM10 antibody
  • IL 2 receptor alpha subunit antibody
  • IL-2 receptor subunit alpha antibody
  • IL-2-RA antibody
  • IL-2R subunit alpha antibody
  • IL2 RA antibody
  • IL2-RA antibody
  • IL2R antibody
  • IL2R, alpha chain antibody
  • IL2RA antibody
  • IL2RA_HUMAN antibody
  • IMD41 antibody
  • Interleukin 2 receptor alpha antibody
  • Interleukin 2 receptor antibody
  • Interleukin-2 receptor subunit alpha antibody
  • p55 antibody
  • t-cell growth factor receptor antibody
  • TAC antibody
  • TAC antigen antibody
  • TCGFR antibody

  see all

Protocols

• Flow cytometry protocols
• Immunoprecipitation protocols