Anti-IL2 Receptor alpha antibody [7D4] (Phycoerythrin) (ab24933)

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Overview

  • Product name
    Anti-IL2 Receptor alpha antibody [7D4] (Phycoerythrin)
    See all IL2 Receptor alpha primary antibodies
  • Description
    Rat monoclonal [7D4] to IL2 Receptor alpha (Phycoerythrin)
  • Host species
    Rat
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    This antibody recognises low affinity IL2 receptor alpha.
  • Tested applications
    Suitable for: Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: IL2 dependent BALB/c mouse helper T cell clone HT2.

  • Epitope
    This antibody reacts with an epitope located between amino acids 125 and 212 of IL2 receptor alpha.
  • Positive control
    • Cells from BALB/c spleen activated with PMA and ionomycin for Immunofluorescent staining with Flow Cytometric analysis.
  • General notes


    This antibody does not block the binding of IL2 to CD25.

Properties

Applications

Our Abpromise guarantee covers the use of ab24933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.

ab154451 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.

 
IP Use at an assay dependent concentration.

Target

  • Function
    Receptor for interleukin-2.
  • Involvement in disease
    Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similarities
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • Interleukin 2 receptor alpha chain antibody
      • CD25 antibody
      • CD25 antigen antibody
      • IDDM10 antibody
      • IL 2 receptor alpha subunit antibody
      • IL-2 receptor subunit alpha antibody
      • IL-2-RA antibody
      • IL-2R subunit alpha antibody
      • IL2 RA antibody
      • IL2-RA antibody
      • IL2R antibody
      • IL2R, alpha chain antibody
      • IL2RA antibody
      • IL2RA_HUMAN antibody
      • IMD41 antibody
      • Interleukin 2 receptor alpha antibody
      • Interleukin 2 receptor antibody
      • Interleukin-2 receptor subunit alpha antibody
      • p55 antibody
      • t-cell growth factor receptor antibody
      • TAC antibody
      • TAC antigen antibody
      • TCGFR antibody
      see all

    Images

    • Flow Cytometry - Anti-IL2 Receptor alpha antibody [7D4] (Phycoerythrin) (ab24933)
      Flow Cytometry - Anti-IL2 Receptor alpha antibody [7D4] (Phycoerythrin) (ab24933)
      Ab24933, at a concentration of 0.1µg for 106 cells, staining CD25 in cells from BALB/c spleen that had been activated by PMA (10 ng/ml) and ionomycin (400 ng/ml) for 48 hours. Stained cells were then analyzed on a flow cytometer.

    References

    ab24933 has not yet been referenced specifically in any publications.

    Publishing research using ab24933? Please let us know so that we can cite the reference in this datasheet.

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