Anti-IL2 Receptor alpha antibody [MRC OX-39] (Biotin) (ab33836)

Overview

  • Product nameAnti-IL2 Receptor alpha antibody [MRC OX-39] (Biotin)
    See all IL2 Receptor alpha primary antibodies
  • Description
    Mouse monoclonal [MRC OX-39] to IL2 Receptor alpha (Biotin)
  • ConjugationBiotin
  • Specificityab33836 recognizes IL2 receptor alpha (55kDa) expressed by activated T cells, but not resting lymphocytes, and by dendritic cells found in the thymus medulla.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Tissue/ cell preparation: Stimulated Rat T cells

  • General notes


    This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate lysine paraformaldehyde). Clone OX 39 has been shown to weakly inhibit the binding of IL2 to Con A stimulated spleen blasts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab33836 was purified from tissue culture supernatant.
  • Primary antibody notesThis clone has been described reacting with paraffin-embedded material following PLP fixation (periodate lysine paraformaldehyde). Clone OX 39 has been shown to weakly inhibit the binding of IL2 to Con A stimulated spleen blasts.
  • ClonalityMonoclonal
  • Clone numberMRC OX-39
  • MyelomaNS0/1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10. use 10µl of the suggested working dilution to label 106 cells.



ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionReceptor for interleukin-2.
  • Involvement in diseaseGenetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similaritiesContains 2 Sushi (CCP/SCR) domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Interleukin 2 receptor alpha chain antibody
    • CD25 antibody
    • CD25 antigen antibody
    • IDDM10 antibody
    • IL 2 receptor alpha subunit antibody
    • IL-2 receptor subunit alpha antibody
    • IL-2-RA antibody
    • IL-2R subunit alpha antibody
    • IL2 RA antibody
    • IL2-RA antibody
    • IL2R antibody
    • IL2R, alpha chain antibody
    • IL2RA antibody
    • IL2RA_HUMAN antibody
    • Interleukin 2 receptor alpha antibody
    • Interleukin 2 receptor antibody
    • Interleukin-2 receptor subunit alpha antibody
    • p55 antibody
    • t-cell growth factor receptor antibody
    • TAC antibody
    • TAC antigen antibody
    • TCGFR antibody
    see all

References for Anti-IL2 Receptor alpha antibody [MRC OX-39] (Biotin) (ab33836)

This product has been referenced in:
  • Whiteland JL  et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-20 (1995). Read more (PubMed: 7868861) »
  • Paterson DJ  et al. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol 24:1281-90 (1987). Read more (PubMed: 2828930) »

See all 2 Publications for this product

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Thank you for contacting us. Please find below the list of the Abcam IL2 receptor alpha antibodies which have been tested in Rat (IL2 receptor alpha antibodies are also known as CD25 antibodies http://www.abcam.com/assets/popups/alternative-names.h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"