The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.1-0.2µg for 106 cells. ab18404-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionReceptor for interleukin-2.
Involvement in diseaseGenetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Flow analysis of IL2 Receptor alpha using ab86911 in ConA-stimulated mouse splenocytes using 0.1 ug/1x106 cells. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-IL2 Receptor alpha antibody. Mouse splenocytes were stimulated with 5 ug/ml ConA for three days prior to testing.
References for Anti-IL2 Receptor alpha antibody [PC61] (FITC) (ab86911)
has not yet been referenced specifically in any publications.
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