The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.06-0.125µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.
FunctionReceptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily. Contains 1 fibronectin type-III domain.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.