Overview

  • Product nameAnti-IL20 antibody
    See all IL20 primary antibodies
  • Description
    Goat polyclonal to IL20
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant full length protein (Human).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.4 (when reconstituted)
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa.
ELISA Use a concentration of 0.5 µg/ml.

Target

  • FunctionCytokine that may be involved in epidermal function and psoriasis. Acts through STAT3.
  • Tissue specificityExpressed at very low levels in skin, trachea, and other tissues.
  • Sequence similaritiesBelongs to the IL-10 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytokine Zcyto10 antibody
    • Four alpha helix cytokine antibody
    • IL-20 antibody
    • IL10D antibody
    • IL20 antibody
    • IL20_HUMAN antibody
    • Interleukin 20 antibody
    • Interleukin-20 antibody
    • MGC96907 antibody
    • UNQ852/PRO1801 antibody
    • ZCYTO10 antibody
    see all

Anti-IL20 antibody images

  • IHC image of ab17426 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17426, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-IL20 antibody (ab17426)

ab17426 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"