Overview

  • Product name
  • Description
    Goat polyclonal to IL21
  • Tested applications
    Suitable for: IHC-P, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: CFQKAQLKSA NTGNNERIIN VSIKKLKRKP PS , corresponding to amino acids 78-109 of Human IL21 Note: Peptide sequence is < 50 % identical to other interleukins in this region.

  • Positive control
    • Human spleen and human tonsil. IHC-P: human hodgkins lymphoma FFPE tissue sections

Properties

Applications

Our Abpromise guarantee covers the use of ab53655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA 1/100000.
ICC 1/500.

Target

  • Function
    Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
  • Tissue specificity
    Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.
  • Sequence similarities
    Belongs to the IL-15/IL-21 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CVID11 antibody
    • IL 21 antibody
    • IL-21 antibody
    • Il21 antibody
    • IL21_HUMAN antibody
    • Interleukin 21 antibody
    • Interleukin-21 antibody
    • interleukin-21 isoform antibody
    • Interleukin21 antibody
    • OTTHUMP00000164088 antibody
    • Za11 antibody
    see all

Images

  • IHC image of IL21 staining in human Hodgkins lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53655, 5µg/ml, for 15 mins at room temperature. A donkey anti-goat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Alam MS  et al. Selective inhibition of the p38 alternative activation pathway in infiltrating T cells inhibits pancreatic cancer progression. Nat Med 21:1337-43 (2015). Read more (PubMed: 26479921) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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