The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 17 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine IL21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Use a concentration of 0.5 - 2 µg/ml. using 100µl/well antibody solution). In conjunction with compatible secondary reagents, Ab77849 allows the detection of at least 0.2 - 0.4 ng/well of recombinant murine IL21.
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL21 (Biotin) (ab77850). Using 100µl/well antibody solution, this antibody can be used as capture antibody with recommended pair.
FunctionCytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
Tissue specificityExpressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.
Sequence similaritiesBelongs to the IL-15/IL-21 family.