Anti-IL21 Receptor antibody [4A9] (ab95711)

Overview

  • Product nameAnti-IL21 Receptor antibody [4A9]
    See all IL21 Receptor primary antibodies
  • Description
    Rat monoclonal [4A9] to IL21 Receptor
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Rat YB2/0 hybridoma transfectant cell line expressing truncated Mouse IL21 Receptor

  • Positive control
    • CD3 stimulated mouse splenocytes

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number4A9
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.



ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionThis is a receptor for interleukin-21.
  • Tissue specificitySelectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 2 fibronectin type-III domains.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    modifications
    C-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD360 antibody
    • IL 21R antibody
    • IL-21 receptor antibody
    • IL-21R antibody
    • IL21 receptor antibody
    • IL21R antibody
    • IL21R_HUMAN antibody
    • Interleukin 21 receptor antibody
    • Interleukin-21 receptor antibody
    • MGC10967 antibody
    • NILR antibody
    • Novel interleukin receptor antibody
    see all

Anti-IL21 Receptor antibody [4A9] images

  • Flow cytometry staining of 2-day Anti-Mouse CD3e stimulated BALB/c splenocytes with Anti-Mouse CD4 APC and 0.25 µg of Rat IgG2a ? Isotype Control (left) or 0.25 µg of ab95711 (right) followed by Anti-Rat IgG Biotin and then Streptavidin PE. Total viable cells were used for analysis.

References for Anti-IL21 Receptor antibody [4A9] (ab95711)

ab95711 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab95711.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"