Anti-IL21 Receptor antibody [4A9] (ab95711)

Overview

  • Product name
    Anti-IL21 Receptor antibody [4A9]
    See all IL21 Receptor primary antibodies
  • Description
    Rat monoclonal [4A9] to IL21 Receptor
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Rat YB2/0 hybridoma transfectant cell line expressing truncated Mouse IL21 Receptor

  • Positive control
    • CD3 stimulated mouse splenocytes

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    4A9
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.



ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    This is a receptor for interleukin-21.
  • Tissue specificity
    Selectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen.
  • Sequence similarities
    Belongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 2 fibronectin type-III domains.
  • Domain
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    modifications
    C-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD360 antibody
    • IL 21R antibody
    • IL-21 receptor antibody
    • IL-21R antibody
    • IL21 receptor antibody
    • IL21R antibody
    • IL21R_HUMAN antibody
    • Interleukin 21 receptor antibody
    • Interleukin-21 receptor antibody
    • MGC10967 antibody
    • NILR antibody
    • Novel interleukin receptor antibody
    see all

Images

  • Flow cytometry staining of 2-day Anti-Mouse CD3e stimulated BALB/c splenocytes with Anti-Mouse CD4 APC and 0.25 µg of Rat IgG2a ? Isotype Control (left) or 0.25 µg of ab95711 (right) followed by Anti-Rat IgG Biotin and then Streptavidin PE. Total viable cells were used for analysis.

References

ab95711 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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