Overview

  • Product nameAnti-IL21 Receptor antibody [4A9] (Biotin)
    See all IL21 Receptor primary antibodies
  • Description
    Rat monoclonal [4A9] to IL21 Receptor (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Rat YB2/0 hybridoma transfectant cell line expressing truncated IL21 Receptor

  • Positive control
    • Mouse splenocytes, thymocytes or bone marrow cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number4A9
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.25-0.5µg for 105-8 cells. in a final volume of 100 µL



ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionThis is a receptor for interleukin-21.
  • Tissue specificitySelectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 2 fibronectin type-III domains.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    modifications
    C-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD360 antibody
    • IL 21R antibody
    • IL-21 receptor antibody
    • IL-21R antibody
    • IL21 receptor antibody
    • IL21R antibody
    • IL21R_HUMAN antibody
    • Interleukin 21 receptor antibody
    • Interleukin-21 receptor antibody
    • MGC10967 antibody
    • NILR antibody
    • Novel interleukin receptor antibody
    see all

Anti-IL21 Receptor antibody [4A9] (Biotin) images

  • Flow cytometry image showing staining of C57BL/6 splenocytes with 0.25 µg of Rat IgG2a ? Isotype Control Biotin (open histogram) or 0.25 µg of ab95712 (filled histogram) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.

References for Anti-IL21 Receptor antibody [4A9] (Biotin) (ab95712)

This product has been referenced in:
  • Vosshenrich CA  et al. Roles for common cytokine receptor gamma-chain-dependent cytokines in the generation, differentiation, and maturation of NK cell precursors and peripheral NK cells in vivo. J Immunol 174:1213-21 (2005). Read more (PubMed: 15661875) »
  • Vincent P Factors influencing patient noncompliance: a theoretical approach. Nurs Res 20:509-16 (1971). Read more (PubMed: 5210829) »

See all 2 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"