The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.25-0.5µg for 105-8 cells. in a final volume of 100 µL
ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
This is a receptor for interleukin-21.
Selectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen.
Belongs to the type I cytokine receptor family. Type 4 subfamily. Contains 2 fibronectin type-III domains.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
C-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame.
Flow cytometry image showing staining of C57BL/6 splenocytes with 0.25 µg of Rat IgG2a ? Isotype Control Biotin (open histogram) or 0.25 µg of ab95712 (filled histogram) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.
References for Anti-IL21 Receptor antibody [4A9] (Biotin) (ab95712)
This product has been referenced in:
Vosshenrich CA et al. Roles for common cytokine receptor gamma-chain-dependent cytokines in the generation, differentiation, and maturation of NK cell precursors and peripheral NK cells in vivo. J Immunol174:1213-21 (2005).
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