Overview

  • Product name
    Anti-IL21 Receptor antibody
    See all IL21 Receptor primary antibodies
  • Description
    Rabbit polyclonal to IL21 Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    NITDQSGNYSQECGS

    , corresponding to amino acids 97-111 of Human IL-21 Receptor.

  • Positive control
    • Raji or A431 whole cell lysate.
  • General notes
    Stable for one year.


    A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. Both IL-21R and the gc are necessary for IL-21 function. IL-21 and its receptor activate the JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. Both IL-21R and the gc are necessary for IL-21 function. IL-21 and its receptor activate the JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 60 kDa.Can be blocked with IL21 Receptor peptide (ab6225).

Target

  • Function
    This is a receptor for interleukin-21.
  • Tissue specificity
    Selectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen.
  • Sequence similarities
    Belongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 2 fibronectin type-III domains.
  • Domain
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    modifications
    C-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD360 antibody
    • IL 21R antibody
    • IL-21 receptor antibody
    • IL-21R antibody
    • IL21 receptor antibody
    • IL21R antibody
    • IL21R_HUMAN antibody
    • Interleukin 21 receptor antibody
    • Interleukin-21 receptor antibody
    • MGC10967 antibody
    • NILR antibody
    • Novel interleukin receptor antibody
    see all

Images

  • Western blot analysis of IL-21R expression in human Raji cell lysate with ab5980 at 1 µg /ml. Western blot analysis of IL-21R expression in human Raji cell lysate with ab5980 at 1 µg /ml.
  • ab5980 at 10µg/ml staining IL-21R in rat lung by IHC

  • Immunofluorescence of IL-21 Receptor in Rat Lung cells using ab5980 at 20 ug/ml.

References

This product has been referenced in:
  • Xing R  et al. Interleukin-21 promotes osteoclastogenesis in RAW264.7 cells through the PI3K/AKT signaling pathway independently of RANKL. Int J Mol Med 38:1125-34 (2016). Read more (PubMed: 27599586) »
  • Wang T  et al. Endothelial interleukin-21 receptor up-regulation in peripheral artery disease. Vasc Med 21:99-104 (2016). Read more (PubMed: 26705256) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab5980.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up