The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa. The detection limit for recombinant Mouse IL22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Use a concentration of 0.5 - 2 µg/ml. ab109819 in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Mouse IL22.
Use a concentration of 2 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of IL22 (50.0 ng/ml).
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL22 (Biotin) (ab84225). ab109819 in conjunction with ab84225 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Mouse IL22.
FunctionCytokine that contributes to the inflammatory response in vivo.
Interleukin 10 related T cell derived inducible factor antibody
interleukin 21 antibody
Interleukin 22 antibody
TIFIL 23 antibody
References for Anti-IL22 antibody (ab109819)
This product has been referenced in:
Graham AC et al. IL-22 production is regulated by IL-23 during Listeria monocytogenes infection but is not required for bacterial clearance or tissue protection. PLoS One6:e17171 (2011).
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