Overview

  • Product name
  • Description
    Rabbit polyclonal to IL22
  • Tested applications
    Suitable for: WB, Indirect ELISA, Neutralising, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant Mouse IL22.

Properties

  • Form
    Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa. The detection limit for recombinant Mouse IL22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. ab109819 in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Mouse IL22.
Neutralising Use a concentration of 2 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of IL22 (50.0 ng/ml).
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL22 (Biotin) (ab84225). ab109819 in conjunction with ab84225 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Mouse IL22.

Target

  • Function
    Cytokine that contributes to the inflammatory response in vivo.
  • Sequence similarities
    Belongs to the IL-10 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytokine Zcyto18 antibody
    • IL 10 related T cell derived inducible factor antibody
    • IL 21 antibody
    • IL 22 antibody
    • IL D110 antibody
    • IL TIF antibody
    • IL-10-related T-cell-derived-inducible factor antibody
    • IL-22 antibody
    • IL-TIF antibody
    • IL21 antibody
    • Il22 antibody
    • IL22_HUMAN antibody
    • ILD110 antibody
    • ILTIF antibody
    • Interleukin 10 related T cell derived inducible factor antibody
    • interleukin 21 antibody
    • Interleukin 22 antibody
    • Interleukin-22 antibody
    • MGC79382 antibody
    • MGC79384 antibody
    • TIFa antibody
    • TIFIL 23 antibody
    • TIFIL23 antibody
    • UNQ3099/PRO10096 antibody
    • zcyto18 antibody
    see all

References

This product has been referenced in:
  • Liu X  et al. The inflammatory cytokine IL-22 promotes murine gliomas via proliferation. Exp Ther Med 13:1087-1092 (2017). Read more (PubMed: 28450947) »
  • Graham AC  et al. IL-22 production is regulated by IL-23 during Listeria monocytogenes infection but is not required for bacterial clearance or tissue protection. PLoS One 6:e17171 (2011). Read more (PubMed: 21347242) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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