Overview

  • Product name
  • Description
    Rabbit polyclonal to IL22
  • Specificity
    The peptide sequence is <50% identical to other interleukins in this region.
  • Tested applications
    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    CRLDKSNFQQPYITNRTFMLAKEASLAD

    , corresponding to amino acids 40-67 of Human IL22 precursor.

  • Positive control
    • Human spleen or tonsil.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 140mM Sodium chloride, 10mM Potassium phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100000.
IHC-P 1/500.

Target

  • Function
    Cytokine that contributes to the inflammatory response in vivo.
  • Sequence similarities
    Belongs to the IL-10 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytokine Zcyto18 antibody
    • IL 10 related T cell derived inducible factor antibody
    • IL 21 antibody
    • IL 22 antibody
    • IL D110 antibody
    • IL TIF antibody
    • IL-10-related T-cell-derived-inducible factor antibody
    • IL-22 antibody
    • IL-TIF antibody
    • IL21 antibody
    • Il22 antibody
    • IL22_HUMAN antibody
    • ILD110 antibody
    • ILTIF antibody
    • Interleukin 10 related T cell derived inducible factor antibody
    • interleukin 21 antibody
    • Interleukin 22 antibody
    • Interleukin-22 antibody
    • MGC79382 antibody
    • MGC79384 antibody
    • TIFa antibody
    • TIFIL 23 antibody
    • TIFIL23 antibody
    • UNQ3099/PRO10096 antibody
    • zcyto18 antibody
    see all

Anti-IL22 antibody images

  • Immunohistochemistry using ab18499 at 1/500 on paraffin-embedded section of human tonsil.

References for Anti-IL22 antibody (ab18499)

This product has been referenced in:
  • Chen JP  et al. IL-22 negatively regulates Helicobacter pylori-induced CCL20 expression in gastric epithelial cells. PLoS One 9:e97350 (2014). IHC-P ; Human . Read more (PubMed: 24824519) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Jujenum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC1 (Tris/EDTA)
Permeabilization
No
Specification
Jujenum
Fixative
Paraformaldehyde
Username

Lina Li

Verified customer

Submitted Jun 05 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Inflammed colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Inflammed colon
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 12 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Mouse Tissue sections (Uterus)
Specification
Uterus
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

Submitted Nov 13 2013

Thank you for contacting me.

I am sorry but you are correct in thinking the Abreview system does not currently support the addition of an Abreview using dot blot. Your customer can do this by submitting the information directly to me (please...

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DISCOUNT CODE: ***
Expiration date: June 7, 2012

I am very pleased to hear you would like to accept our offer and test ab18499 in IHC-Fr. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, ple...

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Vielen Dank für Ihre Email und die Daten mit dem ab18499.
Es tut mir leid zu sehen, dass der ab18499 nicht in gefrorenen Schnitten zu funktionieren scheint. Ich habe mir auch Ihr Protokoll angeschaut, und möchte Ihnen Folgendes noch vorschlagen: <...

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A good alternative to tonsilis spleen and maybe also tissue from the digestive tract, for instance colon or small intestine.

I am sorry to see that your customer is having so much difficulty with so many antibodies. Can you please ask your customer to clarify the fixation step? They listed "xylol" as the fixative, which I expect is just a misunderstanding. Xylol is typically...

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Thank you for contacting us. We would be very interested in your customer testing ab18499 with Dot Blot with human samples. If you could please send me the name, email address and institute of your customer I will create the discount code for you.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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